Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	1700019D03Riktm1(NCOM)Mfgc
Name:	RIKEN cDNA 1700019D03 gene; targeted mutation 1, Mammalian Functional Genomics Centre
MGI ID:	MGI:4880481
Gene:	1700019D03Rik  Location: Chr1:52961483-53031816 bp, - strand  Genetic Position: Chr1, 26.99 cM
Alliance:	1700019D03Riktm1(NCOM)Mfgc page
IMPC:	1700019D03Rik gene page

Mutant Cell Lines:	N01993P1_C_199W_A1, N01993P1_C_199W_B3
Germline Transmission:	Earliest citation of germline transmission: J:204812
Parent Cell Line:	C2 (Tcp) (ES Cell)
Strain of Origin:	C57BL/6N
Project Collection:	NorCOMM

Allele Type:    Targeted (Null/knockout, Reporter) Mutations:    Insertion, Intragenic deletion     Vector: L1L2_NTARU-1   Mutation details: The insertion of NorCOMM cassette pGOHANU created a deletion of size 4113bp between positions 52961200-52965313 of Chromosome 1 (Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an F3 site followed by a universal inverse PCR primer site, splice acceptor IRES-lacZ sequence and a loxP site. This first loxP site is followed by deltaTK and neomycin under the control of the human beta-actin promoter / deltaTK / neomycin phosphotransferase (neoR) gene / SV40 polyA and a second loxP site. After the second loxP site is a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165964)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any 1700019D03Rik Mutation:	57 strains or lines available

Original:	J:165964 Mammalian Functional Genomics Centre, Alleles produced for the NorCOMM project by the Mammalian Functional Genomics Centre (Mfgc), University of Manitoba. MGI Direct Data Submission. 2010;
All:	2 reference(s)