Actb RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:165465 Sehic A, et al., Gene expression and dental enamel structure in developing mouse incisor. Eur J Oral Sci. 2010 Apr;118(2):118-30
Assay type:	RT-PCR
MGI Accession ID:	MGI:4849519
Gene symbol:	Actb
Gene name:	actin, beta

Probe:	Actb-pI, Actb-pJ
Assay notes:	Maxillary incisor tooth germs were removed and cut into incisal tip segment and adjacent segments prior to RNA extraction. These real time RT-PCR data are shown in Table 1.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
P0 tip	P0	TS27: upper jaw incisor	Present	Not Specified; total RNA	CD-1		Not Specified	incisor tip segment.
P0	P0	TS27: upper jaw incisor	Present (a)	Not Specified; total RNA	CD-1		Not Specified	part of the tooth that is adjacent to the incisal tip.
P10 tip	P10	TS28: upper jaw incisor	Present	Not Specified; total RNA	CD-1		Not Specified	incisor tip segment.
P10	P10	TS28: upper jaw incisor	Present (b)	Not Specified; total RNA	CD-1		Not Specified	part of the tooth that is adjacent to the incisal tip.

Notes:
(a) Expression is about 20-fold higher in the adjacent tip fragment than in the incisor tip fragment at this age.
(b) Levels in the adjacent segment were significantly higher than the incisor tip segment.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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