Probe:	Zac1 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Strong		1A
TS15: neural tube	Strong		1A
TS15: pericardial component	Strong		1A
TS15: telencephalon	Strong		1A
TS15: Rathke's pouch	Present	Regionally restricted	1A	Expression is at the entrance of Rathke's pouch.
TS15: trunk	Present	Regionally restricted	1A	Expression is on the thoracic body wall.
TS15: somite	Present		1A
TS15: 1st branchial arch mandibular component	Absent		1A
TS15: forelimb bud	Absent		1A
TS15: hindlimb ridge	Absent		1A

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong		1B
TS15: somite	Present		1B
TS15: hindlimb ridge	Absent		1B

Structure	Level	Pattern	Image	Note
TS20: body wall	Very strong	Regionally restricted	1C	Expression is on the body wall of the umbilical region.
TS20: forelimb pre-cartilage condensation	Very strong		1C	Expression is in the pre-cartilage condensations of the hand and feet.
TS20: hindlimb pre-cartilage condensation	Very strong		1C	Expression is in the pre-cartilage condensations of the hand and feet.
TS20: forelimb digit	Very strong		1C	Expression is in the pre-cartilage condensations.
TS20: hindlimb digit	Very strong		1C	Expression is in the pre-cartilage condensations.
TS20: neural retinal epithelium	Strong		1C
TS20: rib pre-cartilage condensation	Weak		1C

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Strong		1D
TS20: lens vesicle	Absent		1D

Structure	Level	Pattern	Image	Note
TS20: midbrain	Weak		1E
TS20: nasal cavity	Weak		1E
TS20: rib pre-cartilage condensation	Weak		1E
TS20: somite	Weak		1E
TS20: spinal cord	Weak		1E
TS20: telencephalon	Weak		1E
TS20: hindbrain	Absent		1E

Structure	Level	Pattern	Image	Note
TS20: forelimb digit	Very strong	Regionally restricted	1F	Expression is in the pre-cartilage condensations.

Structure	Level	Pattern	Image	Note
TS22: telencephalon ventricular layer	Very strong		2A

Structure	Level	Pattern	Image	Note
TS22: telencephalon ventricular layer	Very strong		2B
TS22: amygdala	Present		2B
TS22: ganglionic eminence	Present		2B
TS22: hippocampus	Absent		2B

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Very strong	Regionally restricted	2C	Expression is in ventral hypothalamic sulcus.
TS22: cerebral aqueduct	Very strong		2C
TS22: neurohypophysis infundibulum	Very strong		2C
TS22: telencephalon	Very strong		2C
TS22: cerebral cortex	Strong	Regionally restricted	2C	Expression is in the ventricular zone of the cingulate cortex.
TS22: diencephalon	Strong	Regionally restricted	2C	Expression is in the thrid ventricle and the mammillothalamic tract area.
TS22: amygdala	Present		2C

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Strong	Regionally restricted	2D	Expression is in the thrid ventricle and the mammillothalamic tract area.

Structure	Level	Pattern	Image	Note
TS22: neurohypophysis infundibulum	Strong		2E

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex ventricular zone	Very strong		2F
TS26: telencephalon ventricular layer	Very strong		2F
TS26: cerebral cortex subventricular zone	Present		2F
TS26: cortical plate	Present		2F
TS26: cerebral cortex marginal zone	Absent		2F

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex ventricular zone	Very strong		2G
TS26: telencephalon ventricular layer	Very strong		2G
TS26: cerebral cortex subventricular zone	Present		2G
TS26: cortical plate	Present		2G
TS26: hippocampus	Weak		2G
TS26: telencephalic part of interventricular foramen	Weak		2G
TS26: cerebral cortex marginal zone	Absent		2G

Structure	Level	Pattern	Image	Note
TS26: diencephalon	Very strong		2H
TS26: cerebral cortex ventricular zone	Very strong		2H

Structure	Level	Pattern	Image	Note
TS26: diencephalon	Very strong		2I
TS26: medulla oblongata	Weak		2I
TS26: thalamus	Weak		2I	Expression is in the geniculate body and in the dorsomedial thalamic nucleus.
TS26: brain	Weak	Regionally restricted	2I	Expression is in the subcommissural organ.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Strong	Regionally restricted	2J	Expression is in the external granular layer.
TS26: cochlear ganglion	Moderate		2J
TS26: midbrain tegmentum	Moderate		2J

Structure	Level	Pattern	Image	Note
TS26: 4th ventricle choroid plexus	Very strong		2K
TS26: cerebral aqueduct	Very strong		2K
TS26: diencephalon	Very strong		2K
TS26: vestibular ganglion	Weak		2K	Expression is in the medial vestibular nucleus.

Structure	Level	Pattern	Image	Note
TS27: brain subventricular zone	Strong		3A
TS27: brain ventricular layer	Strong		3A

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Present		3B
TS28: brain ventricular layer	Present		3B

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Weak		3C
TS28: brain ventricular layer	Weak		3C

Structure	Level	Pattern	Image	Note
TS27: amygdala	Weak		3D
TS27: hippocampus CA3	Weak		3D

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present		3E
TS28: hippocampus CA3	Present		3E

Structure	Level	Pattern	Image	Note
TS28: amygdala	Strong		3F
TS28: hippocampus CA3	Strong		3F

Structure	Level	Pattern	Image	Note
TS27: diencephalon	Present		3G
TS27: dorsomedial hypothalamic nucleus	Strong		3G
TS27: hypothalamus lateral zone	Strong		3G

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Present		3H
TS28: dorsomedial hypothalamic nucleus	Present		3H

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Strong		3I
TS28: dorsomedial hypothalamic nucleus	Weak		3I

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Strong		3J

Structure	Level	Pattern	Image	Note
TS28: hypothalamic nucleus	Present	Regionally restricted	3K	Expression is in the lateral hypothalamic nucleus.

Structure	Level	Pattern	Image	Note
TS28: hypothalamic nucleus	Present	Regionally restricted	3L	Expression is in the lateral hypothalamic nucleus.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		3M
TS28: cerebellum Purkinje cell layer	Very strong		3M
TS28: cerebellum external granule cell layer	Very strong		3M
TS28: cerebellum internal granule cell layer	Present		3M

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3N
TS28: cerebellum internal granule cell layer	Weak		3N
TS28: cerebellum external granule cell layer	Absent		3N

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Moderate		3O
TS28: accessory olfactory bulb	Strong		3O

Structure	Level	Pattern	Image	Note
TS15: hepatic primordium	Very strong
TS15: heart	Strong
TS15: neural tube	Strong
TS15: trunk	Moderate	Regionally restricted		Expression is on the body wall of the umbilical region.
TS15: future midbrain	Moderate
TS15: telencephalon	Moderate
TS15: somite	Trace
TS15: axial skeleton	Absent			Expression is absent in costal cartilage.
TS15: 2nd branchial arch	Absent
TS15: forelimb bud	Absent
TS15: hindlimb ridge	Absent
TS15: optic cup	Absent

Structure	Level	Pattern	Image	Note
TS17: heart	Very strong
TS17: liver	Very strong
TS17: neural tube	Strong
TS17: trunk	Moderate	Regionally restricted		Expression is on the body wall of the umbilical region.
TS17: telencephalon	Moderate
TS17: 2nd branchial arch	Weak
TS17: midbrain	Weak
TS17: somite	Weak
TS17: forelimb bud	Trace
TS17: hindlimb bud	Trace
TS17: optic cup	Trace
TS17: thoracic vertebral pre-cartilage condensation	Absent			Expression is absent in costal cartilage.

Structure	Level	Pattern	Image	Note
TS20: body wall	Very strong	Regionally restricted		Expression is on the body wall of the umbilical region.
TS20: forelimb	Very strong
TS20: hindlimb	Very strong
TS20: retina	Strong
TS20: rib pre-cartilage condensation	Moderate			Expression is in costal cartilage.
TS20: head	Moderate			Expression is in the second branchial arch.
TS20: liver	Moderate
TS20: somite	Moderate
TS20: spinal cord	Moderate
TS20: telencephalon	Moderate
TS20: heart	Weak
TS20: midbrain	Weak

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Very strong
TS22: neurohypophysis infundibulum	Very strong
TS22: diencephalon	Strong	Regionally restricted		Expression is in the neuroepithelium, dorsal diencephalic sulcus, and the mammillothalamic tract area.
TS22: epithalamus	Strong	Regionally restricted		Expression is in the pineal recess.
TS22: cerebral aqueduct	Strong
TS22: choroid plexus	Strong
TS22: midbrain tegmentum	Moderate
TS22: main olfactory bulb	Moderate	Regionally restricted		Expression is in the differentiating field.
TS22: hypothalamus	Moderate	Regionally restricted		Expression is in the arcuate nucleus, dorsomedial hypothalamic nucleus, and the ventromedial hypothalamic nucleus strong in ventral hypothalamic sulcus, weak in the neuroepithelium of periventricular hypothalamic nucleus and trace in preoptic recess.
TS22: thalamus	Weak	Regionally restricted		Expression is in the dorsal thalamus, weak in ventral posterior thalamic nucleus and absent in geniculate body, dorsal lateral and medial and dorsomedial thalamic nucleus, trace in reticular thalamic nucleus.
TS22: pallidum	Weak	Regionally restricted		Expression is in the entopeduncular nucleus.
TS22: amygdala	Weak
TS22: cerebral cortex marginal layer	Weak
TS22: cerebral cortex subventricular zone	Trace
TS22: hindbrain	Trace
TS22: medulla oblongata	Trace	Regionally restricted		Expression is in the medial vestibular nucleus.
TS22: cortical plate	Absent
TS22: hippocampus	Absent
TS22: piriform cortex	Absent
TS22: pons	Absent			Expression is absent in the dorsal cochlear nucleus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Very strong
TS24: cerebral aqueduct	Strong
TS24: choroid plexus	Strong
TS24: neurohypophysis infundibulum	Strong
TS24: main olfactory bulb	Moderate	Regionally restricted		Expression is in the differentiating field.
TS24: midbrain tegmentum	Moderate
TS24: epithalamic recess	Moderate			Absent in the epithalamus area.
TS24: diencephalon	Strong	Regionally restricted		Expression is moderate in the diencephalic sulcus and strong in the neuroepithelium and is absent in the mammillothalamic tract area.
TS24: hypothalamus	Moderate	Regionally restricted		Expression is in the arcuate nucleus, dorsomedial hypothalamic nucleus, and the ventromedial hypothalamic nucleus strong in the ventral hypothalamic sulcus and is weak in the neuroepithelium of periventricular hypothalamic nucleus, preoptic recess.
TS24: amygdala	Weak
TS24: cerebral cortex subventricular zone	Weak
TS24: thalamus	Trace	Regionally restricted		Expression is absent in the dorsal thalamus, trace in geniculate body, dorsal lateral and medial, moderate in the ventral posterior thalamic nucleus and trace in dorsomedial hypothalamic nucleus and is weak in reticular thalamic nucleus.
TS24: cerebral cortex marginal layer	Trace
TS24: cortical plate	Trace
TS24: hindbrain	Trace
TS24: medulla oblongata	Trace	Regionally restricted		Expression is in the medial vestibular nucleus.
TS24: hippocampus	Absent
TS24: piriform cortex	Absent
TS24: pons	Absent			Expression is absent in the dorsal cochlear nucleus.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex ventricular zone	Very strong
TS26: cerebral aqueduct	Strong
TS26: choroid plexus	Strong
TS26: neurohypophysis infundibulum	Strong
TS26: cerebellum	Strong	Regionally restricted		Expression is strong in the external granular layer and is absent in the internal layer.
TS26: cerebral cortex subventricular zone	Moderate
TS26: midbrain tegmentum	Moderate
TS26: main olfactory bulb	Moderate	Regionally restricted		Expression is in the differentiating field.
TS26: diencephalon	Strong	Regionally restricted		Expression is in the neuroepithelium and is moderate in the dorsal diencephalic sulcus and is absent in the mammillothalamic tract area.
TS26: hypothalamus	Moderate	Regionally restricted		Expression is in the arcuate nucleus, dorsomedial hypothalamic nucleus, and the ventromedial hypothalamic nucleus strong in the ventral hypothalamic sulcus and is weak in the neuroepithelium of periventricular hypothalamic nucleus,preoptic recess .
TS26: thalamus	Weak	Regionally restricted		Expression is absent in the dorsal thalamus, weak in geniculate body, dorsal lateral and medial, moderate in the ventral posterior thalamic nucleus and weak in dorsomedial hypothalamic nucleus and reticular thalamic nucleus.
TS26: amygdala	Weak
TS26: cortical plate	Weak
TS26: pons	Weak	Regionally restricted		Expression is in the dorsal cochlear nucleus.
TS26: cerebral cortex marginal zone	Trace
TS26: hindbrain	Trace
TS26: medulla oblongata	Trace	Regionally restricted		Expression is in the medial vestibular nucleus.
TS26: epithalamic recess	Absent
TS26: epithalamus	Absent
TS26: hippocampus CA3	Absent
TS26: piriform cortex	Absent

Structure	Level	Pattern	Image	Note
TS28: dorsomedial hypothalamic nucleus	Strong
TS28: hippocampus CA3	Strong
TS28: accessory olfactory bulb	Moderate
TS28: arcuate nucleus	Moderate
TS28: cerebellum Purkinje cell layer	Moderate
TS28: cerebellum external granule cell layer	Moderate
TS28: choroid plexus	Moderate
TS28: hypothalamic nucleus	Present	Regionally restricted		Expression is strong in the lateral hypothalamic nucleus and weak in the posterior hypothalamic nucleus.
TS28: cerebral cortex	Present	Regionally restricted		Expression is moderate to weak in ventricular and subventricular zones, trace in cortical plate, absent in marginal zone.
TS28: hypothalamus	Weak	Regionally restricted		Expression is in the anterior hypothalamic area.
TS28: main olfactory bulb	Weak	Regionally restricted		Expression is in the differentiating field.
TS28: amygdala	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: medial dorsal thalamic nucleus	Weak
TS28: dentate gyrus granule cell layer	Trace
TS28: dorsal cochlear nucleus	Trace
TS28: hippocampus pyramidal cell layer	Trace
TS28: main olfactory bulb mitral cell layer	Trace
TS28: medulla oblongata	Trace
TS28: ventral posterior thalamic group	Trace
TS28: piriform cortex	Absent

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Very strong
TS28: hippocampus CA3	Very strong
TS28: hypothalamus	Moderate	Regionally restricted		Expression is in the anterior hypothalamic area.
TS28: hypothalamic nucleus	Moderate	Regionally restricted		Expression is in the lateral and posterior hypothalamic nucleus.
TS28: accessory olfactory bulb	Moderate
TS28: amygdala	Moderate
TS28: dorsomedial hypothalamic nucleus	Moderate
TS28: ventromedial hypothalamic nucleus	Moderate
TS28: cerebellum Purkinje cell layer	Weak
TS28: choroid plexus	Weak
TS28: main olfactory bulb mitral cell layer	Weak
TS28: medial dorsal thalamic nucleus	Weak
TS28: medulla oblongata	Weak
TS28: main olfactory bulb	Weak	Regionally restricted		Expression is in the differentiating field.
TS28: cerebral cortex	Trace	Regionally restricted		Expression is trace in ventricular and subventricular zones, absent in cortical plate and marginal zones.
TS28: dentate gyrus granule cell layer	Trace
TS28: hippocampus pyramidal cell layer	Trace
TS28: olfactory nerve	Trace
TS28: piriform cortex	Trace
TS28: telencephalon septum	Trace
TS28: cerebellum external granule cell layer	Absent
TS28: cerebellum internal granule cell layer	Absent
TS28: dorsal cochlear nucleus	Absent
TS28: ventral posterior thalamic group	Absent