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Gene Expression Data
Assay Details
Assay
Reference: J:138858 Georgas K, et al., Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney. Histochem Cell Biol. 2008 Nov;130(5):927-42
Assay type: RNA in situ
MGI Accession ID: MGI:4849244
Gene symbol: Spp1
Gene name: secreted phosphoprotein 1
Probe: MGC clone 6613
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 7Ah: postnatal adult (more )
Structure Level Pattern Image Note
TS28: outer renal medulla Present 7Ah , 7Ai
Specimen 7Aj: postnatal adult (more )
Note: Double labeled: blue - Spp1; orange - Aqp1 (assay MGI:4849228).
Structure Level Pattern Image Note
TS28: outer renal medulla Present 7Aj
Specimen 7Ak: postnatal adult (more )
Note: Double labeled: blue - Spp1; orange - Aqp1 (assay MGI:4849228).
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 7Ak
TS28: loop of Henle Strong 7Ak
Specimen 7Al: postnatal adult (more )
Note: Double labeled: blue - Spp1; orange - Aqp1 (assay MGI:4849228).
Structure Level Pattern Image Note
TS28: premacula segment of distal straight tubule of outer medulla Present 7Al
TS28: loop of Henle thin limb Present Regionally restricted 7Al Expression is present in the ascending and descending thin limb of the loop of Henle as far as the inner stripe of outer medulla.
Specimen 7Am: postnatal adult (more )
Note: Double labeled: blue - Spp1; orange - Aqp1 (assay MGI:4849228).
Structure Level Pattern Image Note
TS28: loop of Henle thin limb Present Regionally restricted 7Am Expression is present in the ascending and descending thin limb of the loop of Henle as far as the inner stripe of outer medulla.
Specimen 7An: postnatal adult (more )
Note: Double labeled: blue - Spp1; orange - Aqp1 (assay MGI:4849228).
Structure Level Pattern Image Note
TS28: premacula segment of distal straight tubule of inner stripe of inner medulla Present 7An
Specimen 7Be: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: medullary renal tubule Present 7Be , 7Bf Expression is present in cortical and medullary tubules of Stage IV nephrons.
TS23: cortical renal tubule Present 7Be , 7Bf Expression is present in cortical and medullary tubules of Stage IV nephrons.
TS23: pelvic urothelial lining Present 7Be
Specimen 7Bg - upper left: embryonic day 15.5 (more )
Note: Double labeled: blue - Spp1; orange - Aqp1 (assay MGI:4849228).
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present Single cells 7Bg left upper Expression is present in a subset of cells.
Specimen 7Bg - lower left: embryonic day 15.5 (more )
Note: Double labeled: blue - Spp1; orange - Aqp1 (assay MGI:4849228).
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present Single cells 7Bg left lower Expression is present in a subset of cells.
Specimen 7Bg - right: embryonic day 15.5 (more )
Note: Double labeled: blue - Spp1; orange - Aqp1 (assay MGI:4849228).
Structure Level Pattern Image Note
TS23: cortical early distal tubule Present 7Bg right
Specimen 7Bh: embryonic day 15.5 (more )
Note: Double labeled: blue - Spp1; orange - Aqp1 (assay MGI:4849228).
Structure Level Pattern Image Note
TS23: immature loop of Henle ascending limb Present 7Bh
TS23: immature loop of Henle descending limb Present Regionally restricted 7Bh Expression is present in the distal portion of the descending limb of the immature loop of Henle.
TS23: medullary collecting duct Weak Single cells 7Bh Expression is present in a subset of cells.
Specimen 7C: embryonic day 15.5 (more )
Note: This schematic summarizes the gene expression data in stage IV nephrons.
Structure Level Pattern Image Note
TS23: cortical early distal tubule Strong 7C Spp1
TS23: immature loop of Henle ascending limb Strong Single cells 7C Spp1 Expression is present in a subset.
TS23: immature loop of Henle descending limb Strong Single cells 7C Spp1 Expression is present in a subset.
TS23: medullary collecting duct Present Single cells 7C Spp1 Expression is present in a subset.
TS23: cortical early proximal tubule Present Single cells 7C Spp1 Expression is present in a subset.
Specimen Not shown: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: pelvic urothelial lining Strong
Specimen 7C: postnatal adult (more )
Note: This schematic summarizes the gene expression data in stage IV nephrons.
Structure Level Pattern Image Note
TS28: thin ascending limb of medulla Strong 7C Spp1
TS28: loop of Henle thin descending limb Strong 7C Spp1
TS28: premacula segment of distal straight tubule of outer medulla Strong 7C Spp1 Expression is present in the distal straight tubule.
TS28: distal convoluted tubule Strong 7C Spp1
TS28: cortical collecting duct Moderate Regionally restricted 7C Spp1 Expression is present in a very small subset of cortical collecting duct tubules in the periphery of the cortex.
Specimen Not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: pelvic urothelial lining Strong
TS28: renal proximal tubule Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory