Antibody:	anti-Aqp1 (AB3065/AB2219)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Some of these specimens are also entries in the GUDMAP database; the single-labled E15.5 specimens are GUDMAP:8202 and the single-labeled adult specimens are GUDMAP:8204.

Structure	Level	Pattern	Image	Note
TS28: renal proximal convoluted tubule	Strong	Regionally restricted	1_Aqp1 low mag , 1m	Expression is present in segment 2 but not 1 of the proximal convoluted tubule.
TS28: proximal straight tubule, cortical portion	Strong		1_Aqp1 low mag , 1n	Expression is present in segment 3.
TS28: loop of Henle thin descending limb	Strong		1_Aqp1 low mag , 1p , 1o
TS28: renal medullary capillary	Present		1o , 1_Aqp1 low mag
TS28: ureteral smooth muscle layer	Present		1_Aqp1 low mag , 1q
TS28: pelvic smooth muscle	Strong		1q , 1_Aqp1 low mag
TS28: renal vasculature	Present	Regionally restricted	1r , 1_Aqp1 low mag	Expression is present in the vessels of the venous system but absent from vessels of the arterial system. It is also present in arterioles, the glomerular capillary system, renal cortical capillaries and medullary capillaries.
TS28: blood	Present		1r , 1_Aqp1 low mag	There was expression present in the erythrocytes.

Structure	Level	Pattern	Image	Note
TS28: blood	Present		2A Aqp1	There was expression present in the erythrocytes.
TS28: glomerular capillary system	Present		2A Aqp1	Expression is present in the endothelial cells of the glomerular capillary system.
TS28: juxtaglomerular complex	Present		2A Aqp1	Expression is present in the endothelial cells of the juxtaglomerular arterioles.
TS28: blood vessel endothelium	Present		2A Aqp1

Structure	Level	Pattern	Image	Note
TS23: cortical early proximal tubule	Strong		3a , 3b
TS23: renal vasculature	Present	Regionally restricted	3b , 3a	Expression is present in the renal cortical capillaries.
TS23: ureteral smooth muscle layer	Present		3a , 3c
TS23: ureteral mesenchyme outer layer	Present		3a , 3c
TS23: pelvic smooth muscle	Present		3c , 3a
TS23: urothelium of ureter	Absent		3c , 3a

Structure	Level	Pattern	Image	Note
TS23: s-shaped body	Present		3d , 3e	Expression is present in endothelial cells invading the cleft of the S-shaped body. Unlike in adults, expression is present in the vessels of the arterial system and not those of the venous system.
TS23: renal vasculature	Present		3d	Expression is present in the vessels of renal cortical arterial system and glomerular capillary system.
TS23: cortical early proximal tubule	Strong		3d

Structure	Level	Pattern	Image	Note
TS25: renal corpuscle	Present	Regionally restricted	3f	Expression is present in the presumptive endothelium of the renal corpuscle.
TS25: cortical early proximal tubule	Absent		3f

Structure	Level	Pattern	Image	Note
TS25: cortical early proximal tubule	Present		3g
TS25: cortical early distal tubule	Absent		3g

Structure	Level	Pattern	Image	Note
TS25: cortical early proximal tubule	Absent		3h	Expression is absent from the presumptive S1 segment of early proximal tubules adjacent to the renal corpuscle its urinary pole.

Structure	Level	Pattern	Image	Note
TS25: cortical early proximal tubule	Present	Regionally restricted	3i	Expression is present in the early proximal tubule, although it is not present in the presumptive S1 segment (the early proximal tubule at the urinary pole of the renal corpuscle).
TS25: renal vasculature	Present		3i	Expression is present in the juxtaglomerular arteriole.
TS25: cortical early distal tubule	Absent		3i

Structure	Level	Pattern	Image	Note
TS25: renal vasculature	Present		3j	Expression is present in the renal medullary capillaries (vasa recta).
TS25: anlage of loop of Henle of cortical renal tubule	Present		3j	Expression is present in the early descending limb of the immature loop of Henle of maturing nephrons in the medulla.

Structure	Level	Pattern	Image	Note
TS25: pelvic smooth muscle	Present
TS25: ureteral smooth muscle layer	Present

Structure	Level	Pattern	Image	Note
TS23: renal vasculature	Present		6B	Expression is present in the endothelial cells which are invading the cleft of the S-shaped body.

Structure	Level	Pattern	Image	Note
TS23: renal vasculature	Present		6C	Expression is present in the renal vasculature, such as the presumptive endothelium, the juxtaglomerular arteriole, the glomerular capillary system, the renal cortical capillary, the renal arterial system, and the renal medullary capillary.
TS23: cortical early proximal tubule	Present		6C
TS23: immature loop of Henle descending limb	Present		6C
TS23: pelvic smooth muscle	Present		6C

Structure	Level	Pattern	Image	Note
TS28: outer renal medulla	Present		7Ac

Structure	Level	Pattern	Image	Note
TS28: segment 2 of proximal tubule	Present	Regionally restricted	7Ad
TS28: segment 1 of proximal tubule	Absent		7Ad
TS28: renal corpuscle	Present	Regionally restricted	7Ad	Expression is present in the glomerular capillary system of the renal corpuscle.
TS28: renal vasculature	Present	Regionally restricted	7Ad	Expression is present in the glomerular capillary system of the renal corpuscle.

Structure	Level	Pattern	Image	Note
TS28: proximal straight tubule, cortical portion	Present		7Ae

Structure	Level	Pattern	Image	Note
TS28: thin descending limb of inner stripe of outer medulla	Present		7Af	Expression is present in the thin limb of the loop of Henle of outer medulla.

Structure	Level	Pattern	Image	Note
TS28: renal vasculature	Present	Regionally restricted	7Ag	Expresion is present in the renal medulary capillary.

Structure	Level	Pattern	Image	Note
TS28: outer renal medulla	Present		7Aj

Structure	Level	Pattern	Image	Note
TS28: renal proximal convoluted tubule	Present		7Ak

Structure	Level	Pattern	Image	Note
TS28: proximal straight tubule, cortical portion	Present		7Al	Expression is present in segment 3.
TS28: thin descending limb of inner stripe of outer medulla	Present		7Al	Expression is present in the thin limb of the loop of Henle.

Structure	Level	Pattern	Image	Note
TS28: thin descending limb of inner stripe of outer medulla	Present		7Am

Structure	Level	Pattern	Image	Note
TS28: renal vasculature	Present		7An	Expression is present in the renal medullary capillary.

Structure	Level	Pattern	Image	Note
TS23: metanephros	Present		7Bc

Structure	Level	Pattern	Image	Note
TS23: immature loop of Henle descending limb	Present		7Bd
TS23: renal vasculature	Present		7Bd	Expression is present in the renal medullary capillary surrounding the tubules.

Structure	Level	Pattern	Image	Note
TS23: cortical early proximal tubule	Present		7Bg left upper

Structure	Level	Pattern	Image	Note
TS23: cortical early proximal tubule	Present		7Bg left lower

Structure	Level	Pattern	Image	Note
TS23: cortical early proximal tubule	Present		7Bg right

Structure	Level	Pattern	Image	Note
TS23: immature loop of Henle descending limb	Present		7Bh	Expression is present in the proximal and distal portion of the descending limb of the immature loop of Henle.