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Gene Expression Data
Assay Details
Assay
Reference: J:138858 Georgas K, et al., Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney. Histochem Cell Biol. 2008 Nov;130(5):927-42
Assay type: RNA in situ
MGI Accession ID: MGI:4849241
Gene symbol: Wnt4
Gene name: wingless-type MMTV integration site family, member 4
Probe: RIKEN clone 2810012H16 subclone 2
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4a: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Calb1 (assay MGI:4849226).
Structure Level Pattern Image Note
TS23: medullary collecting duct Present 4a , 4c
TS23: renal medullary interstitium Present 4a , 4c
Specimen 4d: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Calb1 (assay MGI:4849226).
Structure Level Pattern Image Note
TS23: renal vesicle Present 4d
TS23: pretubular aggregate Present 4d
Specimen 4e: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Calb1 (assay MGI:4849226).
Structure Level Pattern Image Note
TS23: renal vesicle Present 4e
Specimen 4f: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Calb1 (assay MGI:4849226).
Structure Level Pattern Image Note
TS23: renal vesicle Present 4f
TS23: s-shaped body Present 4f
Specimen 4g: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Calb1 (assay MGI:4849226). This is an early nephron.
Structure Level Pattern Image Note
TS23: metanephros Present 4g
Specimen 4h: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Calb1 (assay MGI:4849226). This is a maturing nephron.
Structure Level Pattern Image Note
TS23: metanephros Present 4h
Specimen 4i: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Calb1 (assay MGI:4849226). This is a maturing nephron.
Structure Level Pattern Image Note
TS23: metanephros Present 4i
Specimen 4j: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Calb1 (assay MGI:4849226). This is an early nephron.
Structure Level Pattern Image Note
TS23: s-shaped body Present 4j
Specimen 4k: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Calb1 (assay MGI:4849226).
Structure Level Pattern Image Note
TS23: metanephros Present 4k
Specimen 4n: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Calb1 (assay MGI:4849226).
Structure Level Pattern Image Note
TS23: metanephros Present 4n
Specimen 5a: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Wt1 (assay MGI:4849230).
Structure Level Pattern Image Note
TS23: urothelium of ureter Present 5a
TS23: pretubular aggregate Present 5b , 5a
TS23: early nephron Present 5b , 5a Expression is present in early nephrons.
TS23: s-shaped body Present 5a , 5b
TS23: renal medullary interstitium Present 5c , 5a
TS23: medullary collecting duct Present 5c , 5a
Specimen 5d: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Wt1 (assay MGI:4849230).
Structure Level Pattern Image Note
TS23: pretubular aggregate Present 5d
TS23: renal vesicle Present 5d
Specimen 5e: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Wt1 (assay MGI:4849230).
Structure Level Pattern Image Note
TS23: pretubular aggregate Present 5e
TS23: renal vesicle Present 5e
Specimen 5f: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Wt1 (assay MGI:4849230).
Structure Level Pattern Image Note
TS23: lower limb of comma-shaped body Present 5f
Specimen 5g: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Wt1 (assay MGI:4849230). This is an early S-shaped body.
Structure Level Pattern Image Note
TS23: proximal segment of s-shaped body Present Regionally restricted 5g Expression is present in a small portion of the proximal segment adjacent to the medial segment.
TS23: medial segment of s-shaped body Present 5g
TS23: upper limb of comma-shaped body Present 5g
Specimen 5h: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Wt1 (assay MGI:4849230). This is an S-shaped body.
Structure Level Pattern Image Note
TS23: proximal segment of s-shaped body Present Regionally restricted 5h Expression is present in a small portion of the proximal segment adjacent to the medial segment.
TS23: medial segment of s-shaped body Present 5h
TS23: upper limb of comma-shaped body Present 5h
Specimen 5i: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Wt1 (assay MGI:4849230). This is a late S-shaped body.
Structure Level Pattern Image Note
TS23: proximal segment of s-shaped body Present Regionally restricted 5i Expression is present in a small portion of the proximal segment adjacent to the medial segment.
Specimen 6A: embryonic day 15.5 (more )
Note: Double labeled: blue - Wnt4; orange - Calb1 (assay MGI:4849226).
Structure Level Pattern Image Note
TS23: metanephros Present 6A
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory