Probe:	Pbx1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: limb mesenchyme	Strong	Regionally restricted		Staining is present in the proximal limb.
TS15: limb	Strong	Non-Uniform		Staining is strong in the anterior limb and moderate in the posterior limb.
TS15: neural tube	Strong	Regionally restricted		Staining is present in the lateral neural tube.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Strong	Regionally restricted		Staining is present in the proximal limb.
TS17: limb	Strong	Non-Uniform		Staining is strong in the anterior limb and moderate in the posterior limb.
TS17: liver	Moderate
TS17: 1st branchial arch maxillary component	Strong			Staining is present in the maxillary process.
TS17: genitourinary system	Moderate	Regionally restricted		Staining is present in the external genitalia.
TS17: nasal pit	Moderate			Staining is present in the olfactory placode.
TS17: otocyst	Moderate			Staining is present in the otic pit.
TS17: telencephalon	Strong
TS17: diencephalon	Strong
TS17: midbrain	Strong
TS17: hindbrain	Strong			Staining is present in the metencephalon.
TS17: neural tube	Strong	Regionally restricted		Staining is present in the lateral neural tube.

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Strong	Non-Uniform		Staining is strong in the progress zone and moderate in the middle limb.
TS19: limb	Strong	Non-Uniform		Staining is strong in the interdigital region and moderate in the antero-distal and posterior-distal regions of the limb.
TS19: trunk somite	Strong
TS19: liver	Moderate
TS19: maxillary process	Moderate
TS19: eye	Moderate
TS19: genital tubercle	Strong			Staining is present in the external genitalia.
TS19: telencephalon	Strong
TS19: midbrain	Strong
TS19: hindbrain	Strong			Staining is present in the metencephalon.
TS19: neural tube	Strong	Regionally restricted		Staining is present in the lateral neural tube.