Probe:	Phf5a cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: limb mesenchyme	Moderate	Regionally restricted		Staining is present in the distal limb.
TS15: limb	Moderate			There is moderate staining in the anterior and posterior limb.
TS15: trunk somite	Moderate
TS15: 1st branchial arch maxillary component	Strong			Staining is present in the maxillary process.
TS15: 1st branchial arch	Strong			Staining is present in the mandibular arch.
TS15: 2nd branchial arch	Moderate			Staining is present in the hyoid arch.
TS15: olfactory placode	Strong
TS15: otic pit	Strong

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Strong	Regionally restricted		Staining is present in the progress zone.
TS17: limb	Moderate	Regionally restricted		Staining is present in the anterior limb.
TS17: trunk somite	Moderate
TS17: 1st branchial arch maxillary component	Strong			Staining is present in the maxillary process.
TS17: 1st branchial arch	Moderate			Staining is present in the mandibular arch.
TS17: 2nd branchial arch	Moderate			Staining is present in the hyoid arch.
TS17: nasal pit	Strong			Staining is present in the olfactory placode.
TS17: otocyst	Strong			Staining is present in the otic pit.

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Strong	Non-Uniform		Staining is strong in the progress zone and middle limb and moderate in the proximal limb.
TS19: limb	Strong	Non-Uniform		Staining is strong in the antero-distal and posterior-distal regions and moderate in the antero-proximal region of the limb.
TS19: trunk somite	Moderate
TS19: liver	Moderate
TS19: maxillary process	Strong
TS19: 1st branchial arch	Strong			Staining is present in the mandibular arch.
TS19: genital tubercle	Strong			Staining is present in the external genitalia.
TS19: nasal pit	Strong			Staining is present in the olfactory placode.
TS19: inner ear	Strong			Staining is present in the otic pit.
TS19: hindbrain	Moderate			Staining is present in the metencephalon.