Probe:	Sall4 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: limb mesenchyme	Strong	Regionally restricted		Staining is present in the distal limb.
TS15: limb	Strong	Non-Uniform		Staining is strong in the posterior limb and moderate in the anterior limb.
TS15: tail	Strong
TS15: heart	Strong
TS15: 1st branchial arch maxillary component	Strong			Staining is present in the maxillary process.
TS15: 1st branchial arch	Strong			Staining is present in the mandibular arch.
TS15: eye	Strong
TS15: olfactory placode	Moderate
TS15: otic pit	Moderate
TS15: diencephalon	Strong
TS15: future midbrain	Strong

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Strong	Non-Uniform		Staining is strong in the progress zone and moderate in the middle limb.
TS17: limb	Moderate	Regionally restricted		Staining is present in the posterior limb.
TS17: tail	Strong
TS17: heart	Strong
TS17: 1st branchial arch maxillary component	Strong			Staining is present in the maxillary process.
TS17: 2nd branchial arch	Moderate			Staining is present in the hyoid arch.
TS17: genitourinary system	Strong	Regionally restricted		Staining is present in the external genitalia.
TS17: nasal pit	Strong			Staining is present in the olfactory placode.
TS17: otocyst	Strong			Staining is present in the otic pit.

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Moderate	Regionally restricted		Staining is present in the progress zone.
TS19: limb	Moderate	Regionally restricted		Staining is present in the antero-distal region of the limb.
TS19: tail	Strong
TS19: heart	Moderate
TS19: maxillary process	Moderate
TS19: genital tubercle	Strong			Staining is present in the external genitalia.