Probe:	Mcm4 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: limb mesenchyme	Strong			There is strong staining in the distal and proximal limb.
TS15: limb	Strong			There is strong staining in the anterior and posterior limb.
TS15: telencephalon	Moderate
TS15: diencephalon	Moderate
TS15: future midbrain	Moderate
TS15: future hindbrain	Moderate			Staining is present in the metencephalon.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Strong	Non-Uniform		Staining is strong in the progress zone and moderate in the middle and proximal limb.
TS17: limb	Strong	Non-Uniform		Staining is strong in the posterior limb and moderate in the anterior limb.
TS17: telencephalon	Strong
TS17: diencephalon	Moderate
TS17: midbrain	Strong
TS17: hindbrain	Strong			Staining is present in the metencephalon.

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Strong	Regionally restricted		Staining is present in the progress zone and middle limb.
TS19: limb	Strong	Non-Uniform		Staining is strong in the antero-distal and posterior-distal regions and moderate in the antero-proximal and posterior-proximal regions of the limb.
TS19: telencephalon	Strong
TS19: midbrain	Moderate
TS19: hindbrain	Moderate			Staining is present in the metencephalon.