Gene Expression Data
Assay Details
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Assay
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Reference:
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J:155982
Henry SP, et al., Generation of aggrecan-CreERT2 knockin mice for inducible Cre activity in adult cartilage. Genesis. 2009 Dec;47(12):805-14
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4843377
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Gene symbol:
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Acan
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Gene name:
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aggrecan
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Probe:
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pMAgg-1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2e:
(close  )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * FVB
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Mutant Allele(s):
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Acantm1(cre/ERT2)Crm/Acan+
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Pregnant animal was injected with corn oil on E14.5.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: ulna |
Present |
Regionally restricted |
2e
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Expression is detected in the proliferating chondrocytes, but not in the hypertrophic chondrocytes of the ulna growth plate.
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Specimen
4c:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * FVB
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Mutant Allele(s):
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Acantm1(cre/ERT2)Crm/Acan+
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
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Age:
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postnatal week 2.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: epiphyseal plate |
Present |
Regionally restricted |
4c
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Expression is detected in chondrocytes in the resting zone, proliferating zone and higher hypertrophic zone. No expression was detected in chondrocytes of the lower hypertrophic zone.
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| TS28: knee joint |
Present |
Regionally restricted |
4c
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Expression is detected in chondrocytes in the resting zone, proliferating zone and higher hypertrophic zone. No expression was detected in chondrocytes of the lower hypertrophic zone.
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Specimen
5a:
postnatal month 6; Acantm1(cre/ERT2)Crm/Acan +, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +
(more  )
Note: Animal was injected with oil starting at 6 months for three consecutive days and sacrificed 3 days later.
Specimen
5a:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6 * FVB
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Mutant Allele(s):
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Acantm1(cre/ERT2)Crm/Acan+
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
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Age:
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postnatal month 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Animal was injected with oil starting at 6 months for three consecutive days and sacrificed 3 days later.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: articular cartilage |
Present |
Regionally restricted |
5a
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Expression is present in chondrocytes in the growth plate of the knee joint, in the uncalcified articular cartilage above the tidemark, and in the superficial layer of the meniscus. It is not in chondrocytes in the calcified cartilage below the tidemark.
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| TS28: epiphyseal plate |
Present |
Regionally restricted |
5a
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Expression is present in chondrocytes in the growth plate of the knee joint, in the uncalcified articular cartilage above the tidemark, and in the superficial layer of the meniscus. It is not in chondrocytes in the calcified cartilage below the tidemark.
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| TS28: knee joint |
Present |
Regionally restricted |
5a
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Expression is present in chondrocytes in the growth plate of the knee joint, in the uncalcified articular cartilage above the tidemark, and in the superficial layer of the meniscus. It is not in chondrocytes in the calcified cartilage below the tidemark.
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