Gene Expression Data
Assay Details
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Assay
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Reference:
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J:92504
Harfe BD, et al., Evidence for an expansion-based temporal Shh gradient in specifying vertebrate digit identities. Cell. 2004 Aug 20;118(4):517-28
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4838765
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Results
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Specimen
1C:
embryonic day 10.5
(more  )
Specimen
1C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Graded |
1C
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Staining is observed in a relatively broad gradient in the posterior region of the limb bud.
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Specimen
1E:
embryonic day 11.75
(more )
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
1E
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Staining is observed over a smaller region of the posterior limb bud compared to E10.5.
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Specimen
6E:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Shhtm1(EGFP/cre)Cjt/Shh+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Graded |
6E
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Staining is observed in the posterior portion of the hindlimb bud with a gradient toward the anterior.
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Disp1icb/Disp1tm1Amc
Shhtm1(EGFP/cre)Cjt/Shh+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Graded |
6F
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The range of diffusion of the protein is reduced in mutants.
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Analysis Tools