Probe:	ProTalpha cDNA
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS9: ectoplacental cone	Weak		2A
TS9: primitive streak	Weak	Regionally restricted	2A	Expression is in a small patch of epiblast cells at the anterior of the streak.
TS9: extraembryonic component	Weak	Regionally restricted	2A	Expression is in the chorion and allantois.

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Strong		2B
TS13: trunk unsegmented mesenchyme	Strong		2B
TS13: embryo mesenchyme	Strong		2B	Expression is in intra-embryonic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Strong		2D
TS15: future forebrain	Strong		2D
TS15: future midbrain	Strong	Regionally restricted	2D	Expression is in the midbrain and is absent in the region overlying the midbrain.
TS15: 1st branchial arch	Strong		2E , 2D
TS15: 2nd branchial arch	Strong		2E , 2D
TS15: somite	Strong		2D , 2F	Expression is in posterior somites.
TS15: tail	Strong		2D , 2F	Expression is in posterior somites.
TS15: heart	Absent		2D
TS15: eye	Absent		2D	Absent in the optic eminence.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	2H	Expression is in the area surrounding the auditory vesicle.
TS17: forebrain	Present		2H
TS17: midbrain	Present		2H
TS17: telencephalon	Present		2H
TS17: somite	Weak	Regionally restricted	2H	Absent in anterior somites, low in the dorsal border of posterior somites.
TS17: 1st branchial arch mandibular component	Strong		2H
TS17: 1st branchial arch maxillary component	Strong		2H
TS17: 2nd branchial arch	Strong		2H
TS17: forelimb bud apical ectodermal ridge	Strong		2H
TS17: tail	Strong		2H
TS17: neural tube	Strong	Regionally restricted	2H	Expression is at the dorsal border.
TS17: heart	Absent		2H

Structure	Level	Pattern	Image	Note
TS11: embryo	Weak	Regionally restricted		Expression is in the tailbud and in the presumptive headfold.
TS11: embryo mesoderm	Weak	Regionally restricted		Expression is in non-streak embryonic mesoderm.