Probe:	UI-M-AP1-agh-c-03-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S7_i	Expression is present in the anteroventral hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	S7_j	Expression is present in the anteroventral hypothalamus.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S7_k	Expression is present in the developing suprachiasmatic nucleus.

Structure	Level	Pattern	Image	Note
TS24: hypothalamus	Present	Regionally restricted	S7_l	Expression is present in the developing suprachiasmatic nucleus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_z	Areas of expression include the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_aa	Areas of expression include the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_bb	Areas of expression include the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_cc	Areas of expression include the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Weak	Non-Uniform	Igfbp5-Shh E11.5-coronal , Igfbp5-Shh E11.5-sagittal	There is weak staining in the MZ of the AVH. Staining is either absent or ubiquitous in the MZ of the dorsal thalamus, prethalamus, dorsal & ventral POAs, the PVH, VMH & ARH nuclei, the anterior & posterior IDs, the premam, mam & supramam zones & the TT.
TS19: diencephalon lateral wall ventricular layer	Moderate	Non-Uniform	Igfbp5-Shh E11.5-coronal , Igfbp5-Shh E11.5-sagittal	There is moderate staining in the VZ of the dorsal & ventral POAs, the AVH & anterior & posterior IDs. Staining is either absent or ubiquitous in the VZ of the dorsal thalamus, prethalamus, the PVH, VMH & ARH nuclei, premam, mam & supramam zones & the TT.
TS19: telencephalon mantle layer	Ambiguous		Igfbp5-Shh E11.5-coronal , Igfbp5-Shh E11.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Moderate	Non-Uniform	Igfbp5-Shh E11.5-coronal , Igfbp5-Shh E11.5-sagittal	There is moderate staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Igfbp5-Shh E12.5-sagittal , Igfbp5-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		Igfbp5-Shh E12.5-coronal , Igfbp5-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Igfbp5-Shh E12.5-coronal , Igfbp5-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Ambiguous		Igfbp5-Shh E12.5-coronal , Igfbp5-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer	Ambiguous		Igfbp5-Shh E12.5-coronal , Igfbp5-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence	Moderate	Non-Uniform	Igfbp5-Shh E12.5-sagittal , Igfbp5-Shh E12.5-coronal	There is moderate staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS20: hypothalamus mantle layer	Weak	Non-Uniform	Igfbp5-Shh E12.5-coronal , Igfbp5-Shh E12.5-sagittal	There is weak staining in the mantle zone of the ventral POA. Staining is either absent or ubiquitous in the mantle zone of the dorsal POA, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Strong	Non-Uniform	Igfbp5-Shh E12.5-coronal , Igfbp5-Shh E12.5-sagittal	There is strong staining in the ventricular zone of the anterior and posterior IDs and weak staining in the dorsal POA and the AVH. Staining is either absent or ubiquitous in the ventricular zone of the ventral POA and the PVH, VMH and ARH nuclei.
TS20: thalamus mantle layer	Ambiguous		Igfbp5-Shh E12.5-coronal , Igfbp5-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Ambiguous		Igfbp5-Shh E12.5-coronal , Igfbp5-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Igfbp5-Shh E14.5-coronal , Igfbp5-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		Igfbp5-Shh E14.5-sagittal , Igfbp5-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Igfbp5-Shh E14.5-coronal , Igfbp5-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Ambiguous		Igfbp5-Shh E14.5-sagittal , Igfbp5-Shh E14.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Ambiguous		Igfbp5-Shh E14.5-coronal , Igfbp5-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence	Moderate	Non-Uniform	Igfbp5-Shh E14.5-sagittal , Igfbp5-Shh E14.5-coronal	There is moderate staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS22: hypothalamus mantle layer	Weak	Non-Uniform	Igfbp5-Shh E14.5-sagittal , Igfbp5-Shh E14.5-coronal	There is weak staining in the mantle zone of the anterior ID. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and posterior IDs
TS22: hypothalamus ventricular layer	Strong	Non-Uniform	Igfbp5-Shh E14.5-sagittal , Igfbp5-Shh E14.5-coronal	There is strong staining in the VZ of the ventral POA, moderate staining in the anterior and posterior IDs and weak staining in the dorsal POA and AVH. Staining is either absent or ubiquitous in the VZ of the PVH, VMH and ARH nuclei.
TS22: thalamus mantle layer	Weak	Non-Uniform	Igfbp5-Shh E14.5-sagittal , Igfbp5-Shh E14.5-coronal	There is weak staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS22: thalamus ventricular layer	Strong	Non-Uniform	Igfbp5-Shh E14.5-coronal , Igfbp5-Shh E14.5-sagittal	There is strong staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		Igfbp5-Shh E16.5-sagittal , Igfbp5-Shh E16.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Weak		Igfbp5-Shh E16.5-coronal , Igfbp5-Shh E16.5-sagittal	There is weak staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		Igfbp5-Shh E16.5-sagittal , Igfbp5-Shh E16.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Ambiguous		Igfbp5-Shh E16.5-sagittal , Igfbp5-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer	Ambiguous		Igfbp5-Shh E16.5-sagittal , Igfbp5-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence	Moderate	Non-Uniform	Igfbp5-Shh E16.5-sagittal , Igfbp5-Shh E16.5-coronal	There is moderate staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS24: hypothalamus mantle layer	Very strong	Non-Uniform	Igfbp5-Shh E16.5-coronal , Igfbp5-Shh E16.5-sagittal	There is very strong staining in the mantle zone of the SCH nucleus and moderate staining in the AVH. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, DMH, VMH and ARH nuclei, the DAH and the PH.
TS24: hypothalamus ventricular layer	Moderate	Non-Uniform	Igfbp5-Shh E16.5-coronal , Igfbp5-Shh E16.5-sagittal	There is moderate staining in the VZ of the DMH nucleus and weak staining in the AVH and SCH nucleus. Staining is either absent or ubiquitous in the VZ of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the DAH and the PH.
TS24: thalamus mantle layer	Weak	Non-Uniform	Igfbp5-Shh E16.5-sagittal , Igfbp5-Shh E16.5-coronal	There is weak staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS24: thalamus ventricular layer	Moderate	Non-Uniform	Igfbp5-Shh E16.5-coronal , Igfbp5-Shh E16.5-sagittal	There is moderate staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.