Probe:	Hox-5.3 probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS22: intestine	Present		2C
TS22: spinal cord	Present		2C
TS22: vertebral pre-cartilage condensation	Weak	Regionally restricted	2C	Very weak expression in the pre-vertebra column, restricted to the most peripheral regions of the vertebral body primordia.
TS22: forelimb digit	Present	Regionally restricted	2C	Expression in finger tips.

Structure	Level	Pattern	Image	Note
TS20: vertebral pre-cartilage condensation	Present		2F	Expression in the prevertebra.
TS20: mesonephros	Present	Regionally restricted	2F	Expression in the most posterior part of the mesonephric tissue.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		3G
TS17: hindlimb bud	Present		3G

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	4C	Denser expression in the most distal ends.
TS20: hindlimb	Present	Regionally restricted	4C	Denser expression in the most distal ends.

Structure	Level	Pattern	Image	Note
TS20: humerus pre-cartilage condensation	Absent		4F	No expression in the presumptive humeral head or superficial mesenchyme of the base of the head.

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present	Regionally restricted	4I	Expression in distal region of the forelimb.

Structure	Level	Pattern	Image	Note
TS20: gonad	Present	Regionally restricted	5C	Expression only in the very posterior edge of the indifferent gonad. No expression in the genital ridge.
TS20: mesonephros	Present	Regionally restricted	5C	Expression in the adjacent mesonephros.

Structure	Level	Pattern	Image	Note
TS22: metanephros	Weak	Widespread		Weak, homogeneous signal throughout the metanephros.