Probe:	Hox-5.2 probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS22: intestine	Present		2B
TS22: spinal cord	Present		2B
TS22: vertebral pre-cartilage condensation	Weak	Regionally restricted	2B	Very weak expression in the pre-vertebra column, restricted to the most peripheral regions of the vertebral body primordia.

Structure	Level	Pattern	Image	Note
TS20: vertebral pre-cartilage condensation	Present		2E
TS20: mesonephros	Present	Regionally restricted	2E	Expression in the most posterior part of the mesonephric tissue.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	2I	Expression in the posterior part of the embryo, preferentially in the lateral mesoderm (somatopleure and splanchnopleure).
TS13: somatopleure	Present		2I	Expression in the posterior part of the embryo, preferentially in the lateral mesoderm (somatopleure and splanchnopleure).
TS13: splanchnopleure	Present		2I	Expression in the posterior part of the embryo, preferentially in the lateral mesoderm (somatopleure and splanchnopleure).
TS13: trunk unsegmented mesenchyme	Present	Regionally restricted	2I	Expression in posterior parts of axial presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted		Expression in posterior parts of the neural tube.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Strong		3B
TS15: forelimb bud ectoderm	Absent		3B	No expression in the apical ectodermal layer.

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from somatopleure	Present	Regionally restricted	3D	Expression in both somatopleural and splanchnopleural mesoderm, from forelimb bud to more posterior regions.
TS15: mesenchyme derived from splanchnopleure	Present	Regionally restricted	3D	Expression in both somatopleural and splanchnopleural mesoderm, from forelimb bud to more posterior regions.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3F	Increased expression in a proximal condensation possibly corresponding the the presumptive humeral epiphysis.
TS17: hindlimb bud	Present	Uniform	3F

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	4B	Expression restricted to central regions of condensed cells.
TS20: hindlimb mesenchyme	Present	Regionally restricted	4B	Expression restricted to central regions of condensed cells.

Structure	Level	Pattern	Image	Note
TS20: humerus pre-cartilage condensation	Present	Regionally restricted	4E	Crown-shaped domain of expression around the presumptive humeral head.
TS20: head mesenchyme	Present	Regionally restricted	4E	Restricted expression in more superficial mesenchymal tissue underlying the epidermis on the latero-ventral side of the posterior part of the head.

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present	Regionally restricted	4H	Expression in distal region of the forelimb.
TS22: carpus cartilage condensation	Present	Regionally restricted	4H	Expression at the peripheral of the anlage of the bones of the proximal row of the carpus.
TS22: hand mesenchyme	Present	Regionally restricted	4H	Expression distributed among the whole mesenchyme except for the cartilaginous condensation. Weak expression in a thin mesenchymal layer underlying the epidermis. No expression in perichondrium.

Structure	Level	Pattern	Image	Note
TS22: shoulder joint primordium	Present	Regionally restricted	4K	At the scapulo-humeral junction, expression restricted to the interzone between the humeral and scapular chondrogenic primordia, and to the superficial mesenchyme.
TS22: head mesenchyme	Weak	Regionally restricted	4K	Expression in the lateral thoraco-abdominal and ventro-lateral walls of the base of the head extending until the caudal side of the muzzle.

Structure	Level	Pattern	Image	Note
TS22: humerus cartilage condensation	Present	Regionally restricted	4M	At the distal part of humerus primordium, expression in cells closely surrounding the cartilage bone model. Expression in mesenchymal cells with a fascicular organization neighboring moure roundish unlabelled chondroblasts.

Structure	Level	Pattern	Image	Note
TS22: carpus cartilage condensation	Present	Regionally restricted		Authors report zones of strong expression at the ends of the long bones of both proximal and distal segments, and at the periphery of the anlage of bones of the proximal row of the carpus and tarsus.
TS22: femur cartilage condensation	Present	Regionally restricted		Authors report zones of strong expression at the ends of the long bones of both proximal and distal segments, and at the periphery of the anlage of bones of the proximal row of the carpus and tarsus.
TS22: fibula cartilage condensation	Present	Regionally restricted		Authors report zones of strong expression at the ends of the long bones of both proximal and distal segments, and at the periphery of the anlage of bones of the proximal row of the carpus and tarsus.
TS22: future tarsus	Present	Regionally restricted		Authors report zones of strong expression at the ends of the long bones of both proximal and distal segments, and at the periphery of the anlage of bones of the proximal row of the carpus and tarsus.
TS22: humerus cartilage condensation	Present	Regionally restricted		Authors report zones of strong expression at the ends of the long bones of both proximal and distal segments, and at the periphery of the anlage of bones of the proximal row of the carpus and tarsus.
TS22: radius cartilage condensation	Present	Regionally restricted		Authors report zones of strong expression at the ends of the long bones of both proximal and distal segments, and at the periphery of the anlage of bones of the proximal row of the carpus and tarsus.
TS22: tibia cartilage condensation	Present	Regionally restricted		Authors report zones of strong expression at the ends of the long bones of both proximal and distal segments, and at the periphery of the anlage of bones of the proximal row of the carpus and tarsus.
TS22: ulna cartilage condensation	Present	Regionally restricted		Authors report zones of strong expression at the ends of the long bones of both proximal and distal segments, and at the periphery of the anlage of bones of the proximal row of the carpus and tarsus.

Structure	Level	Pattern	Image	Note
TS20: genital tubercle	Strong	Regionally restricted	5B	Authors report strong expression in the genital ridge until a level more anterior than that seen in the pre-vertebra.
TS20: gonad	Very strong	Regionally restricted	5B	Stronger expression in the cortical zone of the undifferentiated gonad, along the coelomic epithelium and in underlying cells, and clustered in parallel bundles in the medulla.

Structure	Level	Pattern	Image	Note
TS17: mesonephros	Present	Regionally restricted	5G	Expression within tubular epithelium at levels where gonadal labeling occurs; no expression in remaining mesonephric tissue.
TS17: gonad primordium	Present	Regionally restricted	5G	Expression in the presumptive genital ridge.

Structure	Level	Pattern	Image	Note
TS17: mesonephric tubule	Present		5H	Expression within the tubular epithelium at levels where gonadal labeling occurs; no expression in the remaining mesonephric tissue.
TS17: nephric duct	Strong	Regionally restricted	5H	Expression in part of the Wolffian duct.

Structure	Level	Pattern	Image	Note
TS17: gonad primordium	Present	Regionally restricted	5I	Expression in the genital ridge.

Structure	Level	Pattern	Image	Note
TS22: testis mesenchyme	Present		6B	Intense expression in the cortex. Expression in interstitial cells, outside of the seminiferous tubules.
TS22: seminiferous cord	Absent		6B

Structure	Level	Pattern	Image	Note
TS22: ovary	Present	Regionally restricted	6D	Expression distributed throughout the ovarian cortex, with a slight increase in the marginal layers; weaker than in the testis.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Leydig cell	Leydig cell	Present		6F	Expression in interstitial Leydig cells clustered between the seminiferous tubules.

Structure	Level	Pattern	Image	Note
TS28: ovary	Present	Regionally restricted	6H	Expression in interstitial cells of the ovarian cortex.
TS28: ovary follicle	Absent		6H
TS28: ovary stratum granulosum	Absent		6H
TS28: ovary theca	Absent		6H
TS28: oviduct	Absent		6H

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present	Widespread	6J	Expression in the whole metanephric stroma.
TS22: renal tubule	Strong	Regionally restricted	6J	Expression in tubular epithelial structures, and in the ureteric epithelium.
TS22: ureter	Strong	Regionally restricted	6J	Expression in tubular epithelial structures, and in the ureteric epithelium.

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present	Regionally restricted	6L	Expression in metanephric condensation, tubular epithelial cells, and in the ureteric epithelium.
TS22: renal tubule	Present	Regionally restricted	6L	Expression in metanephric condensation, tubular epithelial cells, and in the ureteric epithelium.
TS22: ureter	Present	Regionally restricted	6L	Expression in metanephric condensation, tubular epithelial cells, and in the ureteric epithelium.