Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:19211
Conlon FL, et al., A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse. Development. 1994 Jul;120(7):1919-28
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:4431066
|
|
Gene symbol:
|
Nodal
|
|
Gene name:
|
nodal growth differentiation factor
|
|
Probe:
|
Nodal probe1
|
|
Probe preparation:
|
RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
3A:
embryonic day 5.5
(more  )
Specimen
3A:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 5.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS7: epiblast |
Weak |
Diffuse |
3A
|
Expression in primitive ectoderm; low levels of expression throughout the embryonic ectoderm.
|
Specimen
3B:
embryonic day 6.5
(more )
Note: Early streak stage.
Specimen
3B:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 6.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Early streak stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo ectoderm |
Present |
Regionally restricted |
3B
|
Expression in proximal ectoderm; gradient of expression within the embryonic ectoderm; highest levels in the proximal portion of the embryo.
|
| TS9: primitive endoderm |
Weak |
|
3B
|
Low levels of expression in the overlying visceral endoderm.
|
| TS9: embryo mesoderm |
Absent |
|
3B
|
No detectable expression in the nascent mesodermal cell population.
|
Specimen
3C:
embryonic day 7.0
(more )
Note: Mid-streak stage.
Specimen
3C:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 7.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Mid-streak stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo ectoderm |
Present |
Regionally restricted |
3C
|
Expression continues in the proximal region of the ectoderm, predominantly localized to the proximal-posterior ectoderm.
|
| TS10: primitive endoderm |
Present |
Regionally restricted |
3C
|
Expression in the visceral endoderm overlying the posterior embryonic ectoderm.
|
| TS10: embryo mesoderm |
Absent |
|
3C
|
No expression in mesodermal cells exiting the streak.
|
Specimen
4A:
embryonic day 7.5
(more )
Specimen
4A:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
|
4A
|
Expression localized to the extreme anterior tip of the primitive streak -- the definitive node.
|
Specimen
4C:
embryonic day 7.5
(more )
Note: Early head fold stage.
Specimen
4C:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Early head fold stage.
|
Specimen
4D:
embryonic day 8.0
(more )
Specimen
4D:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo ectoderm |
Present |
Regionally restricted |
4D
|
Expression confined to ectoderm of the node.
|
Specimen
not shown:
embryonic day 8.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo ectoderm |
Present |
Regionally restricted |
|
Expression localized to a small population of ectodermal cell lyting at the lateral borders of the node.
|
Specimen
not shown:
embryonic day 9.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Analysis Tools