Probe:	RP58 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Some embryos were fixed in Bodian's fixative. It is possible another probe (MGI:4431355) was used to obtain some of these results.

Structure	Level	Pattern	Image	Note
TS13: future brain	Absent

Structure	Level	Pattern	Image	Note
TS17: forebrain	Weak	Regionally restricted	2A	There is weak expression in the neuroepithelium.
TS17: neural tube	Weak	Regionally restricted	2A	There is weak expression in the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: diencephalon ventricular layer	Present		2C
TS20: hypothalamus ventricular layer	Present		2C
TS20: thalamus ventricular layer	Present		2C	Expression is present in the ventricular layer of the dorsal thalamus.
TS20: cerebral cortex	Present	Regionally restricted	2C	Expression is present in the entorhinal cortex and the surface region.
TS20: trigeminal ganglion	Present		2C

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		2D	Areas of expression include the surface region.
TS20: olfactory epithelium	Present		2D
TS20: ganglionic eminence	Present		2D

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Weak		2G , 2E
TS21: cortical plate	Strong	Regionally restricted	2G , 2E	There is strong expression in the preplate.
TS21: lens	Weak		2F , 2E
TS21: neural retinal epithelium	Weak		2F , 2E
TS21: olfactory epithelium	Present		2E

Structure	Level	Pattern	Image	Note
TS21: forebrain ventricular layer	Weak		2H

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Very strong		2J , 2I
TS23: cerebellum	Weak		2I
TS23: tectum	Weak		2I

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Strong		3A
TS24: cortical plate	Strong		3A
TS24: hippocampus	Strong		3A
TS24: cerebral cortex ventricular layer	Weak		3A
TS24: corpus striatum	Absent		3A

Structure	Level	Pattern	Image	Note
TS24: amygdala	Strong		3B
TS24: cerebral cortex subventricular zone	Strong		3B
TS24: cortical plate	Strong		3B
TS24: hippocampus	Strong		3B
TS24: cerebral cortex ventricular layer	Weak		3B
TS24: corpus striatum	Absent		3B

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3C	Expression is detected in the lateral amygdaloid nucleus and dorsolateral claustrum but not in the central amygdaloid nuclei.
TS24: basal ganglia	Present	Regionally restricted	3C	Expression is detected in the lateral amygdaloid nucleus and dorsolateral claustrum but not in the central amygdaloid nuclei.

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3D	Expression is detected in the lateral amygdaloid nucleus but not in the central amygdaloid nuclei.

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3E	Expression is detected in the lateral amygdaloid nucleus but not in the central amygdaloid nuclei.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Strong		3G	In the subventricular zone, expression levels are higher in the medial than in the lateral cortical regions.
TS26: hippocampus	Strong		3G
TS26: amygdala	Present		3G	Expression is present in the lateral amygdaloid nucleus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		4A , 4B	Expressing cells include some mitotically active ones.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		4C	Expressing cells include some mitotically active ones.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		4D	Expressing cells include some mitotically active ones.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		4E	Expressing cells include some mitotically active ones.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		4F , 4G	Expressing cells include some mitotically active ones.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		4H	Expressing cells include some mitotically active ones.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		4I	Expressing cells include some mitotically active ones.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Present		4J

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		5A , 5F	Expression is present throughout.
TS28: hippocampus	Present		5A , 5F	Expression is present throughout.
TS28: cerebellum granule cell layer	Present		5A
TS28: main olfactory bulb glomerular layer	Present		5A inset , 5A
TS28: main olfactory bulb granule cell layer	Present		5A inset , 5A
TS28: main olfactory bulb mitral cell layer	Present		5A inset , 5A
TS28: diencephalon	Absent		5A
TS28: midbrain	Absent		5A
TS28: pons	Absent		5A

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		5G , 5B	Expression is present throughout.
TS28: corpus striatum	Absent		5G , 5B

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		5D
TS28: cerebellum Purkinje cell layer	Absent		5D , 5D inset

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		5E , 5H
TS28: cortical layer III	Present		5E , 5H
TS28: cortical layer IV	Present		5E , 5H
TS28: cortical layer V	Present		5E , 5H
TS28: cortical layer VI	Present		5E , 5H
TS28: hippocampus	Present		5E	Expression is present throughout.