H1f8 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:74931 Tanaka M, et al., A mammalian oocyte-specific linker histone gene H1oo: homology with the genes for the oocyte-specific cleavage stage histone (cs-H1) of sea urchin and the B4/H1M histone of the frog. Development. 2001 Mar;128(5):655-64
Assay type:	RT-PCR
MGI Accession ID:	MGI:4429446
Gene symbol:	H1f8
Gene name:	H1.8 linker histone

Probe:	H1foo-pA, H1foo-pB
Assay notes:	cDNA or RNA from 15 oocytes or embryos were used for analyses.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
GV	P (a)	TS28: oocyte	Present	Not Specified; total RNA	Not Specified		Female
MII	P (b)	TS28: oocyte	Present	Not Specified; total RNA	Not Specified		Female
1	E0.5 (c)	TS1: 1-cell stage conceptus	Present	Not Specified; total RNA	Not Specified		Not Specified
2	E1.0 (d)	TS2: 2-cell stage conceptus	Present	Not Specified; total RNA	Not Specified		Not Specified
4	E1.5 (e)	TS3: 4-cell stage conceptus	Weak (f)	Not Specified; total RNA	Not Specified		Not Specified
8	E2.0 (g)	TS3: 8-cell stage conceptus	Absent	Not Specified; total RNA	Not Specified		Not Specified

Notes:
(a) germinal vesicle stage
(b) metaphase II stage oocyte
(c) 1-cell embryos
(d) 2-cell embryos
(e) 4-cell embryos
(f) A substantial decrease in expression was seen after fertilization.
(g) 8-cell embryos

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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