Gad2tm2(cre)Zjh
Targeted Allele Detail
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Symbol: |
Gad2tm2(cre)Zjh |
Name: |
glutamic acid decarboxylase 2; targeted mutation 2, Z Josh Huang |
MGI ID: |
MGI:4418713 |
Synonyms: |
Gad2Cre, Gad2-iCre, Gad2-ires-Cre, GAD65-Cre, GAD65-iCre, GAD65-ires-Cre |
Gene: |
Gad2 Location: Chr2:22512262-22583889 bp, + strand Genetic Position: Chr2, 15.15 cM, cytoband A-B
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Alliance: |
Gad2tm2(cre)Zjh page
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Germline Transmission: |
Earliest citation of germline transmission:
J:151755
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Parent Cell Line: |
v6.5 (ES Cell)
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Strain of Origin: |
(C57BL/6 x 129S4/SvJae)F1
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Project Collection: |
Neuroscience Blueprint cre
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Allele Type: |
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Targeted (Recombinase) |
Mutation: |
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Insertion
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Gad2tm2(cre)Zjh expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Gad2 (MGI:95634) |
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Mutation details: A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the Gad2 (glutamic acid decarboxylase 2) gene. This construct was electroporated into C57BL6/S129 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the selection cassette. The FLPe transgene was subsequently bred out of the background.
(J:151755)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Gad2
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009; |
All: |
280 reference(s) |
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