Summary | Transgene origin | Transgene description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tg(ACTA1-Il15*)11650Lsq
Name:	transgene insertion 11650, LeBris Smith Quinn
MGI ID:	MGI:4417881
Synonyms:	HSA-IL2SP-IL15
Transgene:	Tg(ACTA1-Il15*)11650Lsq  Location: unknown
Alliance:	Tg(ACTA1-Il15*)11650Lsq page

Strain of Origin:

C57BL/6 x C3H

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Tg(ACTA1-Il15*)11650Lsq expresses 1 gene Transgene expresses: Organism Expressed Gene Note mouse Il15 (MGI:103014)   Mutation details: A mouse interleukin-15 (Il15) cDNA sequence encoding the mature endogenous protein (nucleotides 610-951) was modified by removing several post-transcriptional checkpoints that block efficient IL-15 translation and replacing the inefficiently translated and secreted endogenous IL-15 signal peptides with the mouse interleukin-2 (Il2) signal peptide, and stabilizing the COOH terminus of the mature IL-15 protein with a FLAG epitope tag. This IL2SP-IL15 construct was then placed downstream of a modified human alpha-skeletal actin (HSA or ACTA1) promoter fragment. A polyA signal is inserted at the 3' end of the final transgene. The resulting construct was microinjected into C57BL/6 x C3H hybrid oocyte pronuclei. Founder mice were with C57BL/6J mice to generate line 11650 (with a stable single transgene insertion point). IL-15 mRNA/protein expression in skeletal muscle is approximately 1000-fold greater in hemizygous males and approximately 800-fold/1000-fold greater in hemizygous females, respectively. Overexpression in the serum of hemizygous males and females is approximately 300-fold/500-fold, respectively. (J:145611)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:145611 Quinn LS, et al., Oversecretion of interleukin-15 from skeletal muscle reduces adiposity. Am J Physiol Endocrinol Metab. 2009 Jan;296(1):E191-202
All:	2 reference(s)