Probe:	GRIN1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		1A

Structure	Level	Pattern	Image	Note
TS14: embryo	Absent		1B

Structure	Level	Pattern	Image	Note
TS16: cranial ganglion	Present		1C
TS16: facio-acoustic ganglion complex	Present		1C
TS16: glossopharyngeal ganglion	Present		1C
TS16: trigeminal ganglion	Present		1C
TS16: vagus ganglion	Present		1C
TS16: dorsal root ganglion	Present	Regionally restricted	1C	Expression is present at the trunk level.
TS16: future spinal cord	Present	Regionally restricted	1C	Expression is present in the ventral domain.

Structure	Level	Pattern	Image	Note
TS18: midbrain	Present	Regionally restricted	1D	Expression is present in the ventral midbrain.
TS18: future spinal cord	Present		1D	Areas of expression include the dorsolateral region.
TS18: dorsal root ganglion	Present		1D
TS18: acoustic ganglion	Present		1D
TS18: cranial ganglion	Present		1D
TS18: facial ganglion	Present		1D
TS18: glossopharyngeal ganglion	Present		1D
TS18: trigeminal ganglion	Present		1D

Structure	Level	Pattern	Image	Note
TS18: cerebellum primordium	Present		1E	Expression is present in the caudal and rostral rhombic lip.

Structure	Level	Pattern	Image	Note
TS18: neural tube mantle layer	Present	Regionally restricted	1F	Expression is present in the ventral horn.
TS18: dorsal root ganglion	Present		1F

Structure	Level	Pattern	Image	Note
TS20: nervous system	Present	Regionally restricted	2A	Expression is restricted to differentiating cell populations in the nervous system. It is not present in the ventricular zone, the neural crest generating zone, nor in any tissues or organs outside the nervous system.
TS20: telencephalon	Strong	Regionally restricted	2A	There is no expression in the dorsal portion of the telencephalon.
TS20: diencephalon	Strong		2A
TS20: cerebral cortex	Absent		2A
TS20: cranial ganglion	Present		2A
TS20: facial ganglion	Present		2A
TS20: glossopharyngeal ganglion	Present		2A
TS20: trigeminal ganglion	Present		2A
TS20: spinal cord	Present		2A

Structure	Level	Pattern	Image	Note
TS20: nervous system	Present	Regionally restricted	2B	Expression is restricted to differentiating cell populations in the nervous system. It is not present in the ventricular zone, the neural crest generating zone, nor in any tissues or organs outside the nervous system.
TS20: diencephalon	Present		2B
TS20: telencephalon	Present		2B
TS20: vagus ganglion	Present		2B
TS20: cranial ganglion	Present		2B	Expression is present in the cranial ganglia, including XI.
TS20: dorsal root ganglion	Present		2B
TS20: spinal cord	Present		2B

Structure	Level	Pattern	Image	Note
TS20: sympathetic ganglion	Present

Structure	Level	Pattern	Image	Note
TS21: nervous system	Present	Regionally restricted	2C	Expression is restricted to differentiating cell populations in the nervous system. It is not present in the ventricular zone, the neural crest generating zone, nor in any tissues or organs outside the nervous system.
TS21: spinal cord	Present	Regionally restricted	2C	Expression is detected in the whole area of the spinal cord except for the ventricular zone.
TS21: spinal cord ventricular layer	Absent		2C
TS21: telencephalon	Present	Regionally restricted	2C	Expression is not present in the dorsal telencephalon.
TS21: diencephalon	Present		2C
TS21: cranial ganglion	Present		2C
TS21: facial ganglion	Present		2C
TS21: glossopharyngeal ganglion	Present		2C
TS21: trigeminal ganglion	Present		2C
TS21: vestibulo-cochlear ganglion	Present		2C

Structure	Level	Pattern	Image	Note
TS21: nervous system	Present	Regionally restricted	2D	Expression is restricted to differentiating cell populations in the nervous system. It is not present in the ventricular zone, the neural crest generating zone, nor in any tissues or organs outside the nervous system.
TS21: spinal cord	Present	Regionally restricted	2D	Expression is detected in the whole area of the spinal cord except for the ventricular zone.
TS21: spinal cord ventricular layer	Absent		2D
TS21: olfactory lobe	Present	Regionally restricted	2D	Expression is present in the olfactory bulb.
TS21: cranial ganglion	Present		2D
TS21: vagus ganglion	Present		2D
TS21: dorsal root ganglion	Present		2D
TS21: sympathetic ganglion	Present		2D
TS21: retina	Moderate		2D

Structure	Level	Pattern	Image	Note
TS23: nervous system	Present	Regionally restricted	2E	Expression is restricted to differentiating cell populations in the nervous system. It is not present in the ventricular zone, the neural crest generating zone, nor in any tissues or organs outside the nervous system.
TS23: telencephalon	Present		2E	Expression is present in the dorsal and ventral areas.
TS23: diencephalon	Present		2E
TS23: cranial ganglion	Present		2E
TS23: vestibulo-cochlear ganglion	Present		2E
TS23: hindbrain	Present		2E

Structure	Level	Pattern	Image	Note
TS23: nervous system	Present	Regionally restricted	2F	Expression is restricted to differentiating cell populations in the nervous system. It is not present in the ventricular zone, the neural crest generating zone, nor in any tissues or organs outside the nervous system.
TS23: spinal cord	Present	Regionally restricted	2F	Expression is detected in the whole area of the spinal cord except for the ventricular zone.
TS23: spinal cord ventricular layer	Absent		2F
TS23: main olfactory bulb	Present		2F
TS23: cranial ganglion	Present		2F
TS23: vagus ganglion	Present		2F
TS23: retina	Moderate		2F
TS23: dorsal root ganglion	Present		2F
TS23: sympathetic ganglion	Present		2F

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present	Regionally restricted	3A	Expression is present in differentiating fields.
TS23: main olfactory bulb	Weak		3A
TS23: diencephalon	Present	Regionally restricted	3A	Expression is present in the ventral region.
TS23: telencephalon	Present	Regionally restricted	3A	Expression is present in the ventral region.
TS23: cerebral cortex	Present	Regionally restricted	3A	Expression is present in the lateral, but not the medial, cerebral cortex.

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present	Regionally restricted	3B	Expression is present in differentiating fields.
TS23: diencephalon	Present	Regionally restricted	3B	Expression is present in the ventral region.
TS23: telencephalon	Present	Regionally restricted	3B	Expression is present in the ventral region.
TS23: cerebral cortex	Present	Non-Uniform	3B	Expression is present in the lateral, but not the medial, cerebral cortex; there is strong expression in the septum.
TS23: corpus striatum	Strong		3B
TS23: piriform cortex	Strong		3B
TS23: hippocampus	Weak		3B

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present	Regionally restricted	3C	Expression is present in differentiating fields.
TS23: diencephalon	Present	Regionally restricted	3C	Expression is present in the ventral region.
TS23: telencephalon	Present	Regionally restricted	3C	Expression is present in the ventral region.
TS23: thalamus	Strong		3C
TS23: amygdala	Weak		3C
TS23: hippocampus	Weak		3C
TS23: hypothalamus	Weak		3C

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present	Regionally restricted	3D	Expression is present in differentiating fields.
TS23: cerebral cortex	Present	Regionally restricted	3D	Expression is present in the lateral cerebral cortex.
TS23: thalamus	Strong		3D
TS23: hippocampus	Weak		3D
TS23: hypothalamus	Weak		3D
TS23: midbrain	Present		3D	Expression is widely distributed.
TS23: midbrain tegmentum	Moderate		3D

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present	Regionally restricted	3E	Expression is present in differentiating fields.
TS23: superior colliculus	Weak		3E
TS23: midbrain tegmentum	Moderate		3E
TS23: pons	Present	Regionally restricted	3E	Expression is present in the trigeminal motor nucleus.
TS23: midbrain	Present		3E	Expression is widely distributed.
TS23: hindbrain	Present	Regionally restricted	3E	There is no expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present	Regionally restricted	3F	Expression is present in differentiating fields.
TS23: inferior colliculus	Weak		3F
TS23: superior colliculus	Weak		3F
TS23: cerebellum	Moderate		3F
TS23: vestibulo-cochlear ganglion	Strong		3F	Expression is present in the cochlear nucleus.
TS23: midbrain	Present		3F	Expression is widely distributed.
TS23: hindbrain	Present	Regionally restricted	3F	There is no expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present	Regionally restricted	3G	Expression is present in differentiating fields.
TS23: cerebellum	Moderate		3G
TS23: hindbrain	Present	Regionally restricted	3G	Expression is present in the hindbrain, including the precerebellar intramural migration and the posterior precerebellar extramural migration. There is no expression in the ventricular zone.
TS23: midbrain	Present		3G	Expression is widely distibuted.

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present	Regionally restricted	3H	Expression is present in differentiating fields.
TS23: spinal cord	Strong	Regionally restricted	3H	Expression is detected in the whole area of the spinal cord except for the ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Absent
TS23: ganglionic eminence	Absent
TS23: hindbrain ventricular layer	Absent
TS23: midbrain ventricular layer	Absent
TS23: spinal cord ventricular layer	Absent
TS23: pons	Present	Non-Uniform		There is moderate expression in portions of the pons. There is only trace expression in the anterior pons.
TS23: medulla oblongata	Moderate
TS23: hindbrain	Present			Expression is present in yet-to-be identified nuclei.

Structure	Level	Pattern	Image	Note
TS25: central nervous system	Present	Regionally restricted	3I	Expression is present in differentiating fields.
TS25: main olfactory bulb	Present		3I
TS25: telencephalon	Present	Regionally restricted	3I	Expression is present in almost all areas of the forebrain.
TS25: cerebral cortex	Present		3I	Expression is present in the medial and lateral cortex.

Structure	Level	Pattern	Image	Note
TS25: central nervous system	Present	Regionally restricted	3J	Expression is present in differentiating fields.
TS25: telencephalon	Present	Regionally restricted	3J	Expression is present in almost all areas of the forebrain.
TS25: cerebral cortex	Present	Non-Uniform	3J	Expression is present in the cerebral cortex. There is strong expression in the septum.
TS25: corpus striatum	Strong		3J
TS25: piriform cortex	Strong		3J

Structure	Level	Pattern	Image	Note
TS25: central nervous system	Present	Regionally restricted	3K	Expression is present in differentiating fields.
TS25: telencephalon	Present	Regionally restricted	3K	Expression is present in almost all areas of the forebrain.
TS25: cerebral cortex	Present		3K
TS25: thalamus	Strong		3K
TS25: amygdala	Moderate		3K
TS25: hypothalamus	Moderate		3K
TS25: hippocampus	Present		3K
TS25: olfactory lobe	Present	Regionally restricted	3K	Expression is present in the lateral migratory stream and the nucleus of the lateral olfactory tract.

Structure	Level	Pattern	Image	Note
TS25: central nervous system	Present	Regionally restricted	3L	Expression is present in differentiating fields.
TS25: amygdala	Moderate		3L
TS25: hypothalamus	Moderate		3L
TS25: hippocampus	Present		3L
TS25: midbrain tegmentum	Strong	Regionally restricted	3L	There is strong expression in the red nucleus.
TS25: periaqueductal grey	Strong		3L
TS25: midbrain	Present		3L

Structure	Level	Pattern	Image	Note
TS25: central nervous system	Present	Regionally restricted	3M	Expression is present in differentiating fields.
TS25: inferior colliculus	Moderate		3M
TS25: midbrain tegmentum	Moderate		3M
TS25: superior colliculus	Moderate		3M
TS25: pons	Present	Non-Uniform	3M	Expression is present throughout the pons, including the anterior pons. There is strong expression in the pontine nucleus.
TS25: midbrain	Present		3M
TS25: hindbrain	Moderate	Regionally restricted	3M	There is no expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS25: central nervous system	Present	Regionally restricted	3N	Expression is present in differentiating fields.
TS25: inferior colliculus	Moderate		3N
TS25: superior colliculus	Moderate		3N
TS25: midbrain	Present		3N	Expression in the present in the midbrain, including in the tegmental nucleus.
TS25: pons	Present	Regionally restricted	3N	Expression is present in the locus coeruleus, trigeminal nucleus complex, the facial motor nucleus, and the superior olivary nucleus.
TS25: vestibulo-cochlear ganglion	Present		3N	Expression is present in the cochlear nucleus.
TS25: hindbrain	Moderate	Regionally restricted	3N	There is no expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS25: central nervous system	Present	Regionally restricted	3O	Expression is present in differentiating fields.
TS25: inferior colliculus	Moderate		3O
TS25: cerebellum	Moderate		3O
TS25: hindbrain	Present	Regionally restricted	3O	Moderate expression is present in the hindbrain; strong expression is present in the anterior precerebellar extramural migration. There is no expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS25: central nervous system	Present	Regionally restricted	3P	Expression is present in differentiating fields.
TS25: spinal cord	Strong	Regionally restricted	3P	Expression is detected in the whole area of the spinal cord except for the ventricular zone.
TS25: spinal cord ventricular layer	Absent		3P

Structure	Level	Pattern	Image	Note
TS25: ganglionic eminence	Absent
TS25: hindbrain ventricular layer	Absent
TS25: midbrain ventricular layer	Absent
TS25: telencephalon ventricular layer	Absent

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb granule cell layer	Strong		4A
TS27: main olfactory bulb mitral cell layer	Strong		4A

Structure	Level	Pattern	Image	Note
TS27: forebrain	Present	Non-Uniform	4B	Expression is strong in lateral and dorsal areas but is reduced in the ventral portion of the forebrain.
TS27: cerebral cortex	Strong		4B
TS27: piriform cortex	Strong		4B
TS27: corpus striatum	Weak		4B
TS27: telencephalon septum	Weak		4B

Structure	Level	Pattern	Image	Note
TS27: forebrain	Present	Non-Uniform	4C	Expression is strong in lateral and dorsal areas but is reduced in the ventral portion of the forebrain.
TS27: amygdala	Strong		4C
TS27: cerebral cortex	Strong		4C
TS27: hippocampus	Strong		4C
TS27: thalamus	Strong		4C
TS27: hypothalamus	Present	Non-Uniform	4C	Expression is high in the dorsal hypothalamus but is signficantly reduced in lateral and anterior hypothalamus.
TS27: paraventricular hypothalamic nucleus	Strong		4C
TS27: zona incerta	Strong		4C

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Strong		4D

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Weak		4E
TS27: pontine nucleus	Strong		4E
TS27: midbrain tegmentum	Weak		4E
TS27: periaqueductal grey	Weak		4E
TS27: superior colliculus	Weak		4E

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Weak		4F
TS27: inferior colliculus	Weak		4F

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Weak		4G

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Present		4H	Expression levels are greaty reduced from what was seen before.

Structure	Level	Pattern	Image	Note
TS27: medulla oblongata	Trace
TS27: pons	Trace
TS27: brain ventricular layer	Absent

Structure	Level	Pattern	Image	Note
TS28: forebrain	Present	Regionally restricted	5A	Expression is restricted to where newly born neurons are maturing.
TS28: main olfactory bulb granule cell layer	Strong		5A
TS28: main olfactory bulb mitral cell layer	Strong		5A

Structure	Level	Pattern	Image	Note
TS28: forebrain	Present	Regionally restricted	5B	Expression is restricted to where newly born neurons are maturing.
TS28: piriform cortex	Strong		5B

Structure	Level	Pattern	Image	Note
TS28: forebrain	Present	Regionally restricted	5C	Expression is restricted to where newly born neurons are maturing.
TS28: hippocampal formation	Strong		5C
TS28: cerebral cortex	Weak		5C
TS28: amygdala	Weak		5C
TS28: basolateral amygdaloid nucleus	Weak		5C
TS28: lateral amygdaloid nucleus	Weak		5C
TS28: medial amygdaloid nucleus	Weak		5C
TS28: dentate gyrus	Strong		5C

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		5D

Structure	Level	Pattern	Image	Note
TS28: hindbrain	Trace		5E	Hardly any expression is detected.
TS28: midbrain	Trace		5E	Hardly any expression is detected.
TS28: cerebellum granule cell layer	Weak		5E

Structure	Level	Pattern	Image	Note
TS28: hindbrain	Trace		5F	Hardly any expression is detected.
TS28: midbrain	Trace		5F	Hardly any expression is detected.
TS28: cerebellum granule cell layer	Weak		5F

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Weak		5G
TS28: hindbrain	Trace	Regionally restricted	5G	Hardly any expression is detected. No expression is detected in the ventricular zone of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Absent		5H	Virtually no signal is detected.