Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:76619
Barlow GM, et al., Mammalian DSCAMs: roles in the development of the spinal cord, cortex, and cerebellum?. Biochem Biophys Res Commun. 2002 May 10;293(3):881-91
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:4356119
|
Gene symbol:
|
Dscam
|
Gene name:
|
DS cell adhesion molecule
|
Probe:
|
CHD2M-14
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
2Aa:
embryonic day 12.5
(more )
Note: This is a sagittal section.
Specimen
2Aa:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a sagittal section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Graded |
2Aa
|
Ventral expression predominates.
|
Specimen
2Bf:
embryonic day 12.5
(more )
Note: This is a transverse section at the level of the heart and forelimbs.
Specimen
2Bf:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a transverse section at the level of the heart and forelimbs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Graded |
2Bf
|
Ventral expression predominates along the rostro-caudal axis of the spinal cord.
|
Specimen
2Bk:
embryonic day 12.5
(more )
Note: This is a transverse section at the level of the liver and hindlimbs.
Specimen
2Bk:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a transverse section at the level of the liver and hindlimbs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Graded |
2Bk
|
Ventral expression predominates along the rostro-caudal axis of the spinal cord.
|
Specimen
3a:
embryonic day 10.5
(more )
Note: This is a transverse section.
Specimen
3a:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a transverse section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Regionally restricted |
3a
|
Expression is present in the ventral neural tube, where it is seen in the motor neuroblasts.
|
Specimen
3c:
embryonic day 11.5
(more )
Note: This is a transverse section.
Specimen
3c:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a transverse section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Strong |
Regionally restricted |
3c
|
Expression is present in the developing motor neurons of the ventral neural tube.
|
Specimen
3e:
embryonic day 12.5
(more )
Note: This is a transverse section.
Specimen
3e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a transverse section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Graded |
3e
|
Expression in the motor cells of the ventral spinal cord predominates.
|
Specimen
Not shown:
embryonic day 15.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: spinal cord |
Present |
Graded |
|
Expression in the motor cells of the ventral spinal cord predominates.
|
Specimen
3g:
embryonic day 18.5
(more )
Note: This is a transverse section.
Specimen
3g:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a transverse section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Present |
Uniform |
3g
|
Expression is present throughout the spinal cord, with no distinct ventral/dorsal gradient.
|
Specimen
3i:
postnatal adult
(more )
Note: This is a transverse section.
Specimen
3i:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a transverse section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spinal cord |
Strong |
Non-Uniform |
3i
|
Expression is somewhat stronger in the ventral regions.
|
Specimen
Not shown:
embryonic day 11.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
4a:
embryonic day 12.5
(more )
Note: This is a sagittal section.
Specimen
4a:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a sagittal section.
|
Specimen
4c:
embryonic day 14.5
(more )
Note: This is a sagittal section.
Specimen
4c:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a sagittal section.
|
Specimen
4e:
postnatal day 1
(more )
Note: This is a sagittal section.
Specimen
4e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a sagittal section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: neocortex layer |
Present |
Non-Uniform |
4e
|
Expression is present in all cortical layers but is more strongly expressed in the midlayers.
|
TS27: cingulate cortex |
Present |
Regionally restricted |
4e
|
Expression is present in the retrosplenial granular cortex.
|
Specimen
4g:
postnatal day 1
(more )
Note: This is a coronal section.
Specimen
4g:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a coronal section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: neocortex layer |
Present |
Non-Uniform |
4g
|
Expression is present in all cortical layers but is more strongly expressed in the midlayers.
|
TS27: cingulate cortex |
Present |
Regionally restricted |
4g
|
Expression is present in the retrosplenial granular cortex.
|
Specimen
5a:
postnatal month 6
(more )
Note: This is a sagittal section.
Specimen
5a:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal month 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a sagittal section.
|
Specimen
5e:
postnatal day 7
(more )
Note: This is a sagittal section.
Specimen
5e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a sagittal section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: main olfactory bulb |
Strong |
Regionally restricted |
5e
|
There is strong expression in the neuronal layers.
|
Specimen
6a:
postnatal newborn
(more )
Note: This is a sagittal section.
Specimen
6a:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a sagittal section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cerebellum |
Present |
|
6a
|
Structural definition is not yet distinct.
|
Specimen
6c:
postnatal adult
(more )
Note: This is a sagittal section.
Specimen
6c:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a sagittal section.
|
|