Summary | Mutation origin | Mutation description | Expression | Recombinase activity | Phenotypes | Find Mice (IMSR) | References

Symbol:	Pax7tm2.1(cre/ERT2)Fan
Name:	paired box 7; targeted mutation 2.1, Chen-Ming Fan
MGI ID:	MGI:4353154
Synonyms:	Pax7CE, Pax7Cre-ERT2
Gene:	Pax7  Location: Chr4:139464373-139560839 bp, - strand  Genetic Position: Chr4, 70.83 cM
Alliance:	Pax7tm2.1(cre/ERT2)Fan page

Germline Transmission:	Earliest citation of germline transmission: J:150962
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Inducible, Null/knockout, Recombinase) Inducer:    tamoxifen Mutations:    Insertion, Intragenic deletion   Pax7tm2.1(cre/ERT2)Fan expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Pax7 (MGI:97491)   Mutation details: Beginning with the Pax7tm1.2Sjc allele in which exon 2 was knocked out leaving a single loxP site, part of exon 1 was replaced with a cre/ERT2 (ESR1) fusion followed by an IRES-DsRed and an frt flanked neo cassette. Germ line, flp mediated recombination removed the neo cassette. The absence of full-length Pax7 protein expression was confirmed by western blot analysis on P10 tibialis anterior muscle extracts. Expression of the fusion cre/ERT2 protein was confirmed by a cross with a reporter line. DsRed fluorescence was not detectable by epifluorescence nor was DsRed protein by immunostaining. (J:150962)

Activity:	Tissue activity of this recombinase allele
Driver:	Pax7 (mouse) Summary of all recombinase alleles driven by Pax7. Comparative matrix view of recombinase activities Alliance mouse Pax7 gene page

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pax7 Mutation:	39 strains or lines available

Original:	J:150962 Lepper C, et al., Adult satellite cells and embryonic muscle progenitors have distinct genetic requirements. Nature. 2009 Jul 30;460(7255):627-31
All:	104 reference(s)