Gene Expression Data
Assay Details
Assay
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Reference:
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J:144618
Weichenhan D, et al., A mouse translocation associated with Caspr5-2 disruption and perinatal lethality. Mamm Genome. 2008 Dec;19(10-12):675-86
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3851416
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Gene symbol:
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Cntnap5b
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Gene name:
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contactin associated protein-like 5B
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Probe:
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Caspr5-2 is2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Beta-galactosidase
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Assay notes:
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The authors state that qualitatively similar results were obtained with the other oligo probes: Caspr5-2 is1 (MGI:3851354) or Caspr5-2 is3 (MGI:3851356). The probe used in this assay was 3' labelled.
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Results
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Specimen
6A,B:
embryonic day 15.5
(more )
Note: (B) is a higher magnification of the head are shown in (A)
Specimen
6A,B:
(close )
Genetic Background:
|
NMRI
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Age:
|
embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
|
Note:
|
(B) is a higher magnification of the head are shown in (A)
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: hindbrain |
Present |
Non-Uniform |
6A WT Caspr5-2
,
6B
|
Expression is weak at the isthmus.
|
TS23: midbrain |
Present |
Non-Uniform |
6A WT Caspr5-2
,
6B
|
Expression is weak at the isthmus.
|
TS23: cerebral cortex |
Strong |
|
6A WT Caspr5-2
,
6B
|
|
TS23: cortical plate |
Strong |
|
6A WT Caspr5-2
,
6B
|
|
TS23: forebrain |
Strong |
|
6A WT Caspr5-2
,
6B
|
|
TS23: hypothalamus |
Strong |
|
6A WT Caspr5-2
,
6B
|
|
TS23: tectum |
Strong |
|
6A WT Caspr5-2
,
6B
|
|
TS23: hippocampus |
Weak |
|
6B
,
6A WT Caspr5-2
|
|
TS23: medulla oblongata |
Weak |
|
6B
,
6A WT Caspr5-2
|
|
TS23: midbrain tegmentum |
Weak |
|
6B
,
6A WT Caspr5-2
|
|
TS23: spinal cord |
Weak |
|
6B
,
6A WT Caspr5-2
|
|
Specimen
6C:
postnatal adult
(more )
Specimen
6C:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal adult
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
|