Probe:	Tbx5 cDNA5
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Strong		1K	Strong expression throughout the forelimb bud.
TS15: hindlimb ridge	Absent		1K	No expression in the region of the hindlimb primordium.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present		1L	Authors state that differential expression between Tbx4 and Tbx5 in limb mesenchyme is maintained.
TS17: hindlimb bud	Absent		1L	Authors state that differential expression between Tbx4 and Tbx5 in limb mesenchyme is maintained.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	1M	Marked proximodistal boundary of expression.
TS19: handplate	Weak	Regionally restricted	1M	Expression in the distal tip of the autopod is greatly reduced.
TS19: hindlimb bud	Absent		1M

Structure	Level	Pattern	Image	Note
TS20: hand mesenchyme	Present	Regionally restricted	1N	Expression only in the mesenchyme surrounding the cartilage primordia of digits I and V.
TS20: interdigital region between forelimb digits 1 and 2 mesenchyme	Present		1N	Expression only in the mesenchyme surrounding the cartilage primordia of digits I and V.
TS20: hindlimb	Absent		1N

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	1O	Expression only in the mesenchyme surrounding the cartilage primordia of digits I and V.
TS21: interdigital region between forelimb digits 1 and 2 mesenchyme	Present		1O	Expression only in the mesenchyme surrounding the cartilage primordia of digits I and V.
TS21: interdigital region between forelimb digits 4 and 5 mesenchyme	Present		1O	Expression only in the mesenchyme surrounding the cartilage primordia of digits I and V.
TS21: hindlimb	Absent		1O

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	2D	Marked proximodistal boundary of expression.
TS19: handplate	Weak	Regionally restricted	2D	Expression in the distal tip of the autopod is greatly reduced.
TS19: handplate apical ectodermal ridge	Absent		2D

Structure	Level	Pattern	Image	Note
TS14: forelimb bud mesenchyme	Present	Regionally restricted	3A	Expression in the lateral plate mesoderm overlying somites 6-12 (the prospective forelimb field) in a 14 somite embryo.
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted	3A	Expression in the lateral plate mesoderm overlying somites 6-12 (the prospective forelimb field) in a 14 somite embryo.