Probe:	Gaa cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Single cells	2B	Expression is in pyramidal and granule neurons.
TS28: meninges	Moderate	Single cells	2B	Expression is in the meningeal cells of the pia mater-arachnoid.
TS28: hippocampus molecular layer	Weak	Single cells	2B

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong	Single cells	2C
TS28: choroid plexus	Strong		2C	Expression is in cells lining the choroid plexus.

Structure	Level	Pattern	Image	Note
TS28: inferior colliculus	Strong	Regionally restricted	2D	Expression is in neurons adjacent to the cerebral aqueduct located in the inferior colliculus nucleus and dorsal tegmental nuclei.
TS28: midbrain tegmentum	Strong	Regionally restricted	2D	Expression is in neurons adjacent to the cerebral aqueduct located in the inferior colliculus nucleus and dorsal tegmental nuclei.
TS28: white matter	Weak	Regionally restricted	2D	Expression is in glial cells of the white matter.
TS28: brain ependyma	Weak	Regionally restricted	2D	Expression is in ependymal lining cells.

Structure	Level	Pattern	Image	Note
TS28: brainstem	Strong	Single cells	2E	Expression is in neurons.
TS28: cerebellum Purkinje cell layer	Strong	Single cells	2E	Expression is in neurons.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Moderate		2F

Structure	Level	Pattern	Image	Note
TS28: endometrium stroma	Weak	Diffuse	2H
TS28: endometrium glandular epithelium	Weak		2H

Structure	Level	Pattern	Image	Note
TS28: ventricle cardiac muscle	Moderate	Diffuse	2J

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Weak	Regionally restricted	2L	Expression is in myocytes in paraspinal muscle.

Structure	Level	Pattern	Image	Note
TS24: hypothalamus	Moderate	Regionally restricted	2M	Expression is in differentiating neurons.

Structure	Level	Pattern	Image	Note
TS24: inner ear epithelium	Moderate	Regionally restricted	2N	Expression is in the epithelial lining cells.

Structure	Level	Pattern	Image	Note
TS24: sympathetic ganglion	Moderate		2O

Structure	Level	Pattern	Image	Note
TS24: seminiferous cord	Moderate	Regionally restricted	2P	Expression is in epithelial cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: telencephalon		Present	Non-Uniform		Expression is moderate to high in neurons throughout, moderate in pia mater-arachnoid meningeal cells and in choroid plexus lining epithelial cells, and is very low in ependymal cells, very low to low in glial cells/white matter.
TS28: midbrain		Present	Non-Uniform		Expression is moderate to high in neurons/colliculus and tegmental nuclei, very low to low in glial cells/white matter.
TS28: cerebellum Purkinje cell layer		Strong
TS28: cerebellum		Present	Non-Uniform		Expression is low to moderate in other neurons aside from the Purkinje cells, and is very low to low in glial cells/white matter.
TS28: brainstem		Present	Non-Uniform		Expression is moderate to high in neurons, very low to low in glial cells/white matter.
TS28: spinal cord ventral horn		Strong
TS28: sympathetic ganglion		Strong
TS28: spinal cord		Weak	Non-Uniform		Expression is very low to low in glial cells/white matter.
TS28: eye		Trace	Non-Uniform		Expression is in cells of ganglion, internuclear, and outer layers, and in extraocular myocytes.
TS28: cardiac muscle tissue		Weak
TS28: cerebellum granule cell layer		Weak
TS28: cerebellum molecular layer		Weak
TS28: cerebellum white matter		Weak
TS28: endometrium stroma		Weak
TS28: heart atrium		Weak
TS28: heart ventricle		Weak
TS28: lamina propria of vagina		Weak
TS28: Sertoli cell	Sertoli cell	Moderate
TS28: endometrium glandular epithelium		Trace
TS28: endometrium luminal epithelium		Trace
TS28: interstitium of the testis		Trace
TS28: myometrium		Trace
TS28: spermatogonium		Trace
TS28: stomach		Trace
TS28: epithelium of epididymis		Moderate
TS28: epithelium of vagina		Trace	Regionally restricted		Expression is in glandular epithelial cells.
TS28: cortical renal tubule		Weak	Regionally restricted		Expression is in cortical tubulaar epithelial cells.
TS28: renal medulla		Trace	Regionally restricted		Expression is in medullar tubular epithelial cells.
TS28: ganglion		Weak	Regionally restricted		Expression is in neurons and intestinal ganglia.
TS28: intestine epithelium		Trace	Regionally restricted		Expression is in stromal cells and in mucosal epithelial cells.
TS28: intestine mucosa		Trace	Regionally restricted		Expression is in stromal cells and in mucosal epithelial cells.
TS28: colon		Trace	Regionally restricted		Expression is in the colon lamina propria.
TS28: liver		Weak	Regionally restricted		Expression is weak in hepatocytes and is absent in cells of ducts and portal area.
TS28: adrenal gland		Trace	Diffuse
TS28: lung		Trace	Diffuse
TS28: pancreas		Trace	Diffuse
TS28: spleen		Trace	Diffuse
TS28: thymus		Trace	Diffuse
TS28: skeletal muscle		Weak			Expression is in myocytes. Included sections of the quadriceps, anterior tibialis, triceps, biceps, diaphragm, tongue, paraspinal, and extraocular muscle.
TS28: heart valve		Absent

Structure	Level	Pattern	Image	Note
TS15: embryo	Trace	Ubiquitous

Structure	Level	Pattern	Image	Note
TS20: embryo	Trace	Ubiquitous

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Weak	Ubiquitous
TS22: embryo	Weak	Non-Uniform		Expression is very low to low in tissues outside the central nervous system.

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Moderate	Regionally restricted		Expression is weak to moderate in differentiating neurons.
TS24: inner ear	Strong	Regionally restricted		Expression is in the epithelial lining cells.
TS24: peripheral nervous system	Strong	Regionally restricted		Expression is in neurons.
TS24: sympathetic ganglion	Strong
TS24: hypothalamus	Moderate
TS24: seminiferous cord	Moderate
TS24: intestine	Weak
TS24: skeletal muscle	Present	Regionally restricted		Expression is very low to low in myocytes in most muscle groups, moderate in myocytes of head and neck muscles.
TS24: atrium cardiac muscle	Trace
TS24: ventricle cardiac muscle	Trace
TS24: embryo	Trace	Regionally restricted		Expression was very low to low in most cell types.