Gene Expression Data
Assay Details
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Assay
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Reference:
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J:138310
Xu C, et al., Expression of Robo/Slit and Semaphorin/Plexin/Neuropilin family members in the developing hypothalamic paraventricular and supraoptic nuclei. Gene Expr Patterns. 2008 Sep;8(7-8):502-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3845292
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Gene symbol:
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Plxna1
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Gene name:
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plexin A1
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Probe:
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Plxna1 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Fixative was Methancarn (60% methanol,
30% chloroform, and 10% acetic acid).
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Results
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Specimen
1D1,D5:
embryonic day 12.5
(more  )
Note: (D5) is a higher magnification of the boxed area in (D1).
Specimen
1D1,D5:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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(D5) is a higher magnification of the boxed area in (D1).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus |
Present |
Regionally restricted |
1_D1
,
1_D5
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Expression is in the paraventricular nucleus/supraoptic nucleus progenitor region.
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Specimen
1D2:
embryonic day 13.5
(more )
Specimen
1D2:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus |
Present |
Regionally restricted |
1_D2
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Expression becomes reciprocally regulated in the paraventricular nucleus/supraoptic nucleus versus their surrounding regions.
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Specimen
1D3:
embryonic day 14.5
(more )
Specimen
1D3:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hypothalamus |
Present |
Regionally restricted |
1_D3
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Expression becomes reciprocally regulated in the paraventricular nucleus/supraoptic nucleus versus their surrounding regions.
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Specimen
1D4:
embryonic day 15.5
(more )
Specimen
1D4:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Present |
Regionally restricted |
1_D4
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The lowest level of expression is at the medial half of the paraventricular nucleus (PVN) and at the supraoptic nucleus, and its highest level of expression is just ventral to the PVN.
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Analysis Tools