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Gene Expression Data
Assay Details
Assay
Reference: J:78343 Loebel DA, et al., Isolation of differentially expressed genes from wild-type and Twist mutant mouse limb buds. Genesis. 2002 Jul;33(3):103-13
Assay type: RNA in situ
MGI Accession ID: MGI:3842754
Gene symbol: Ptn
Gene name: pleiotrophin
Probe: 3-1(1E)
Probe preparation: labelled with digoxigenin RNA
Results
Specimen 3f +/+: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: limb mesenchyme Present Regionally restricted 3f +/+ top , 3f +/+ bottom , 3f +/+ middle Expression was detected in the proximal mesenchyme and in the mesenchyme immediately subjacent to the apical ectoderm.
TS17: head Present Regionally restricted 3f +/+ top Expression was detected in the craniofacial region.
TS17: branchial arch Present 3f +/+ top
TS17: neural tube Present 3f +/+ top
Specimen 3f -/-: embryonic day 10.5; Twist1tm1Bhr/Twist1tm1Bhr (more )
Structure Level Pattern Image Note
TS17: head Absent 3f -/- top Expression was lost from the craniofacial region and the caudal segment of the neural tube.
TS17: neural tube Absent 3f -/- top Expression was lost from the craniofacial region and the caudal segment of the neural tube.
TS17: branchial arch Absent 3f -/- top
TS17: forelimb bud Present Regionally restricted 3f -/- top , 3f -/- middle Expression intensity was comparable to the wild type, but the expressing cells occupied a proportionally larger area of the limb bud.
TS17: hindlimb bud Weak Regionally restricted 3f -/- bottom , 3f -/- top Expression was reduced in the mutant, but the expression domain in the proximal limb bud mesenchyme was similar to the wild type hindlimb bud.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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