Probe:	mNFI-X cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS14: embryo	Absent

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	3X	Expression is present in the neocortex.
TS19: brain ventricular layer	Present		3X
TS19: 1st branchial arch	Present		3X	Expression is present throughout the mandibular arch.
TS19: liver	Present		3X
TS19: lung	Present		3X

Structure	Level	Pattern	Image	Note
TS19: trunk myotome	Present			Expression is present in skeletal muscles.
TS19: neural tube	Present	Regionally restricted		Expression is present in the ependymal layer of the neural tube.
TS19: limb mesenchyme	Present	Regionally restricted		Expression is present in central and proximal mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Non-Uniform		Expression was the same as was described for E11.5.
TS20: skeletal muscle	Present	Regionally restricted		Expression is present in the developing skeletal muscles of the limbs and trunk.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Non-Uniform	4X	Expression was the same as was described for E11.5.
TS22: limb musculature	Present		4X
TS22: skeletal muscle	Present	Regionally restricted	4X	Expression is present in the developing skeletal muscles of the trunk.
TS22: embryo mesenchyme	Present	Regionally restricted	4X	Expression is present around umbilical blood vessels.
TS22: gut mesentery	Present		4X
TS22: neocortex	Very strong		4X
TS22: cerebellum	Present	Regionally restricted	4X	Strong expression is present in migrating neurons.
TS22: spinal cord	Present	Regionally restricted	4X	Strong expression is present in migrating neurons.

Structure	Level	Pattern	Image	Note
TS22: embryo mesenchyme	Present	Regionally restricted		Expression is present around umbilical blood vessels.
TS22: gut mesentery	Present
TS22: genital tubercle	Present

Structure	Level	Pattern	Image	Note
TS23: tooth	Present			Expression is present in tooth primordia.

Structure	Level	Pattern	Image	Note
TS24: tooth	Present		5X	Expression is present in tooth primordia.
TS24: pons	Strong		5X	There is high expression in pontine nuclei.
TS24: cerebral cortex	Strong		5X
TS24: skin	Present	Regionally restricted	5X	Expression is present around the whisker follicles.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	6X	Expression is predominantly in the gray matter, suggesting it is expressed by neurons.
TS28: dentate gyrus	Present		6X	Expression levels in the dentate gyrus are higher than in the horn of Ammon cells.
TS28: hippocampus	Present		6X

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Present

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present