Gene Expression Data
Assay Details
Assay
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Reference:
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J:113766
Zhang M, et al., Foxj1 regulates asymmetric gene expression during left-right axis patterning in mice. Biochem Biophys Res Commun. 2004 Nov 26;324(4):1413-20
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3841415
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Probe:
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Nodal probe10
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4A:
embryonic day 8.0
(more )
Note: This is an early somite stage embryo.
Specimen
4A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is an early somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: lateral plate mesoderm |
Present |
Regionally restricted |
4A
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In 5 of 5 embryos examined there was expression in the left lateral plate mesoderm but not the right.
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TS12: node |
Present |
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4A
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Specimen
4B:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Foxj1tm1Bph/Foxj1tm1Bph
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Age:
|
embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
This is an early somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: lateral plate mesoderm |
Present |
|
4B
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In 2 of 6 cases examined expression was present in both the left and right lateral plate mesoderm.
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TS12: node |
Present |
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4B
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There was bilateral expression in the node.
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Specimen
4C:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Foxj1tm1Bph/Foxj1tm1Bph
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Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
This is an early somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: lateral plate mesoderm |
Absent |
|
4C
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In 2 of 6 embryos examined there was no expression in either the left or right lateral plate mesoderm.
|
TS12: node |
Present |
|
4C
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There was bilateral expression in the node.
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Specimen
4D:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Foxj1tm1Bph/Foxj1tm1Bph
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Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
This is an early somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: lateral plate mesoderm |
Absent |
|
4D
|
In 2 of 6 embryos examined there was no expression in either the left or right lateral plate mesoderm.
|
TS12: node |
Absent |
|
4D
|
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Specimen
Not shown:
embryonic day 8.0; Foxj1tm1Bph/ Foxj1tm1Bph
(more )
Note: This is an early somite stage embryo.
Specimen
Not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * Black Swiss
|
Mutant Allele(s):
|
Foxj1tm1Bph/Foxj1tm1Bph
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
This is an early somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: lateral plate mesoderm |
Present |
Regionally restricted |
|
In 1 of 6 embryos examined there was expression in the left lateral plate mesoderm but not the right.
|
Specimen
Not shown:
embryonic day 8.0; Foxj1tm1Bph/ Foxj1tm1Bph
(more )
Note: This is an early somite stage embryo.
Specimen
Not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * Black Swiss
|
Mutant Allele(s):
|
Foxj1tm1Bph/Foxj1tm1Bph
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
This is an early somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: lateral plate mesoderm |
Present |
Regionally restricted |
|
In 1 of 6 embryos examined there was expression in the right lateral plate mesoderm but not the left.
|
Specimen
Not shown:
embryonic day 8.0; Foxj1tm1Bph/Foxj1 +
(more )
Note: This is an early somite stage embryo.
Specimen
Not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * Black Swiss
|
Mutant Allele(s):
|
Foxj1tm1Bph/Foxj1+
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Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
This is an early somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: lateral plate mesoderm |
Present |
Regionally restricted |
|
In 8 of 8 embryos examined there was expression in the left lateral plate mesoderm but not the right.
|
|