Sprr2d RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:147038 Lee HJ, et al., Novel markers of early ovarian pre-granulosa cells are expressed in an Sry-like pattern. Dev Dyn. 2009 Apr;238(4):812-25
Assay type:	RNA in situ
MGI Accession ID:	MGI:3840614
Gene symbol:	Sprr2d
Gene name:	small proline-rich protein 2D

Probe:	Sprr2d cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1E XX Sprr2d ©

Specimen 1E XX: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: ovary	Present	Regionally restricted	1E XX Sprr2d	Expression is in a subset of cells with a higher concentration at the posterior end.

1E XY Sprr2d ©

Specimen 1E XY: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: testis	Present	Single cells	1E XY Sprr2d	Expression is in a few cells at the very posterior end.

Specimen Not shown XX: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: mesonephros	Absent
TS21: ovary	Absent

Specimen Not shown XY: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: mesonephros	Absent
TS21: testis	Absent

2_Sprr2d ©

Specimen 2: embryonic day 11.75 (more

)
Note: embryo was bisected down the midline of the neural tube

Structure	Level	Pattern	Image	Note
TS20: ovary	Present		2_Sprr2d	Expression was found only in the gonads.

3A 12 ts XX ©

Specimen 3A XX 12 ts: embryonic day 11.0 (more

)
Note: 12 tail somites.

Structure	Level	Pattern	Image	Note
TS18: gonad primordium	Present	Regionally restricted	3A 12 ts XX	Expression is in a distinct subset of cells in the central gonadal region that overlies the anterior mesonephric tubules.
TS18: intraembryonic coelom	Absent		3A 12 ts XX	Absent in the the coelomic epithelium.

3A 12 ts XY ©

Specimen 3A XY 12 ts: embryonic day 11.0 (more

)
Note: 12 tail somites.

Structure	Level	Pattern	Image	Note
TS18: gonad primordium	Present	Regionally restricted	3A 12 ts XY	Expression is in a distinct subset of cells inthe central gonadal region that overlies the anterior mesonephric tubules.
TS18: intraembryonic coelom	Absent		3A 12 ts XY	Absent in the the coelomic epithelium.

3A 15-16 ts XX ©

Specimen 3A XX 15-16 ts: embryonic day 11.25 (more

)
Note: 15-16 tail somites.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present	Regionally restricted	3A 15-16 ts XX	Expression is in a distinct subset of cells in the central gonadal region that overlies the anterior mesonephric tubules.
TS19: intraembryonic coelom	Absent		3A 15-16 ts XX	Absent in the the coelomic epithelium.

3A 15-16 ts XY ©

Specimen 3A XY 15-16 ts: embryonic day 11.25 (more

)
Note: 15-16 tail somites.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present	Regionally restricted	3A 15-16 ts XY	Expression is in a distinct subset of cells in the central gonadal region that overlies the anterior mesonephric tubules.
TS19: intraembryonic coelom	Absent		3A 15-16 ts XY	Absent in the the coelomic epithelium.

3A 18 ts XX ©

Specimen 3A XX 18 ts: embryonic day 11.5 (more

)
Note: 18 tail somites.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present	Regionally restricted	3A 18 ts XX	Expression is in the central gonadal region that overlies the anterior mesonephric tubules.
TS19: intraembryonic coelom	Absent		3A 18 ts XX	Absent in the the coelomic epithelium.

3A 18 ts XY ©

Specimen 3A XY 18 ts: embryonic day 11.5 (more

)
Note: 18 tail somites.

Structure	Level	Pattern	Image	Note
TS19: gonad	Weak	Regionally restricted	3A 18 ts XY	Expression is in the central gonadal region that overlies the anterior mesonephric tubules.
TS19: intraembryonic coelom	Absent		3A 18 ts XY	Absent in the the coelomic epithelium.

3A 20 ts XX ©

Specimen 3A XX 20 ts: embryonic day 11.6 (more

)
Note: 20 tail somites.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present		3A 20 ts XX	Expression is throughout the length of the gonad.
TS19: intraembryonic coelom	Absent		3A 20 ts XX	Absent in the the coelomic epithelium.

3A 20 ts XY ©

Specimen 3A XY 20 ts: embryonic day 11.6 (more

)
Note: 20 tail somites.

Structure	Level	Pattern	Image	Note
TS19: gonad	Weak		3A 20 ts XY	Expression is throughout the length of the gonad.
TS19: intraembryonic coelom	Absent		3A 20 ts XY	Absent in the the coelomic epithelium.

3A 21 ts XX ©

Specimen 3A XX 21 ts: embryonic day 11.75 (more

)
Note: 21 tail somites.

Structure	Level	Pattern	Image	Note
TS20: ovary	Strong	Regionally restricted	3A 21 ts XX	Expression is in a distinct subset of cells throughout the length of the gonad.

3A 21 ts XY ©

Specimen 3A XY 21 ts: embryonic day 11.75 (more

)
Note: 21 tail somites.

Structure	Level	Pattern	Image	Note
TS20: testis	Weak	Graded	3A 21 ts XY	Expression is decreased anteriorly.

3A 23 ts XX ©

Specimen 3A XX 23 ts: embryonic day 12.0 (more

)
Note: 23 tail somites.

Structure	Level	Pattern	Image	Note
TS20: ovary	Strong	Regionally restricted	3A 23 ts XX	Expression is in a distinct subset of cells throughout the length of the gonad.

3A 23 ts XY ©

Specimen 3A XY 23 ts: embryonic day 12.0 (more

)
Note: 23 tail somites.

Structure	Level	Pattern	Image	Note
TS20: testis	Weak	Graded	3A 23 ts XY	Expression is decreased anteriorly.

3A 26 ts XX ©

Specimen 3A XX 26 ts: embryonic day 12.25 (more

)
Note: 26 tail somites.

Structure	Level	Pattern	Image	Note
TS20: ovary	Present	Graded	3A 26 ts XX	Expression is decreased anteriorly.

3A 26 ts XY ©

Specimen 3A XY 26 ts: embryonic day 12.25 (more

)
Note: 26 tail somites.

Structure	Level	Pattern	Image	Note
TS20: testis	Weak	Regionally restricted	3A 26 ts XY	There is a small amount of expression at the posterior pole.

3A E12.75 XX ©

Specimen 3A XX E12.75: embryonic day 12.75 (more

)

Structure	Level	Pattern	Image	Note
TS20: ovary	Weak	Graded	3A E12.75 XX	Expression is decreased anteriorly.

3A E12.75 XY ©

Specimen 3A XY E12.75: embryonic day 12.75 (more

)

Structure	Level	Pattern	Image	Note
TS21: testis	Absent		3A E12.75 XY

4_Sprr2d +/+ ©

Specimen 4 +/+: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: ovary	Present	Graded	4_Sprr2d +/+	There is a posterior bias in expression.

Specimen 4 Wv/Wv: embryonic day 12.5; Kit^W-v/Kit^W-v (more

)

Structure	Level	Pattern	Image	Note
TS20: ovary	Present		4_Sprr2d Wv/Wv	Expression in this mutant is the same as in wild-type embryos.

Specimen 6B Wnt4+/+: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: ovary	Present		6B Sprr2d +/+

Specimen 6B Wnt4-/-: embryonic day 12.5; Wnt4^tm1Amc/Wnt4^tm1Amc (more

)

Structure	Level	Pattern	Image	Note
TS20: ovary	Present		6B Sprr2d -/-	Expression in this mutant is the same as in wild-type.

Specimen Not shown Wnt4+/-: embryonic day 12.5; Wnt4^tm1Amc/Wnt4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS20: ovary	Present			Expression in this heterozygote is the same as in wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24