Probe:	Wnt3a probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Regionally restricted	2B	Expression in the dorsal portion of regions of the neural tube.
TS17: neural tube roof plate	Present		2B
TS17: hindbrain	Present	Regionally restricted	2B
TS17: forebrain	Present	Regionally restricted	2B	Dorsal midline expression bifurcates at the telencephalic flexure.
TS17: body wall mesenchyme	Present	Regionally restricted	2B	Expression in the mesenchyme tissue surrounding the umbilical veins.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	2D	Expression in small areas of the bilateral developing telencephalic bulbs.

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present	Regionally restricted	3B	Expression in a narrow band along the dorsal midlne. Expression extends nearly to the rostral tip of the neuraxis.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	3E	Strong expression in areas just dorsolateral to the telencephalic flexure.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	3H	Strong expression in areas just dorsolateral to the telencephalic flexure.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	3K	Expression in a small area at the boundary between the dorsal and ventral thalamus. Expression surrounds the myelencephalic roof.

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present	Regionally restricted	3N	Strong expression in areas just dorsolateral to the telencephalic flexure.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	4B	Highest expression around the dorsal portion of the telencephalic flexures.
TS20: thalamus	Present	Regionally restricted	4B	Small area of expression marking the boundary between the dorsal and ventral thalamus. No expression in ventral thalamus structures.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	4D	Expression in the dorsal midline up to the diencephalon.
TS20: telencephalon	Present	Regionally restricted	4D	Line of expression bifurcates to extend into the medial walls of the telencephalon.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4F	Line of expression bifurcates to extend into the medial walls of the telencephalon.
TS20: choroid invagination	Present	Regionally restricted	4F	Expression surrounding lateral choroid plexus

Structure	Level	Pattern	Image	Note
TS20: spinal cord roof plate	Present		5B	Expression confined to a triangle-shaped groups of cells that form the presumptive roof plate.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Present	Regionally restricted	5D	Band of midline expression bifurcates to accommodate the myencephalic roof.
TS20: medulla oblongata roof	Present	Regionally restricted	5D	Expression surrounding posterior choroid plexus.

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Regionally restricted	5F	Authors report general expression throughout the ectoderm. Relatively low levels of expression in ectoderm covering the backs.
TS20: limb epithelium	Present	Regionally restricted	5F	Higher levels of expression in ectoderm covering the limb buds
TS20: mandibular process	Present	Regionally restricted	5F	Higher levels of expression in the caudal position of ectoderm covering the mandibular arch.
TS20: oral cavity	Present	Regionally restricted	5F	Expression extends slightly into the oral cavity.

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Absent			No expression in the epithelium lining the oral cavity.