Probe:	T31 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Cell Type	Level	Pattern	Image	Note
TS28: seminiferous tubule		Present	Regionally restricted	5a	Expression was detected in the outer layer of seminiferous tubules.
TS28: spermatogonium		Present		5b inset , 5a , 5b	Expression was detected in spermatogonia and Sertoli cells located adjacent to the basement membrane of the seminiferous tubules and in Leydig cells. No differentiating spermatocytes or spermatids hybridized with the probe.
TS28: Leydig cell	Leydig cell	Present		5b inset , 5b , 5a
TS28: Sertoli cell	Sertoli cell	Present		5b , 5a , 5b inset
TS28: spermatid		Absent		5b inset , 5a , 5b	Expression was detected in spermatogonia and Sertoli cells located adjacent to the basement membrane of the seminiferous tubules and in Leydig cells. No differentiating spermatocytes or spermatids hybridized with the probe.
TS28: spermatocyte		Absent		5b inset , 5a , 5b	Expression was detected in spermatogonia and Sertoli cells located adjacent to the basement membrane of the seminiferous tubules and in Leydig cells. No differentiating spermatocytes or spermatids hybridized with the probe.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Present		5d