Gene Expression Data
Assay Details
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Assay
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Reference:
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J:108509
Blaess S, et al., Sonic hedgehog regulates Gli activator and repressor functions with spatial and temporal precision in the mid/hindbrain region. Development. 2006 May;133(9):1799-809
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3810918
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Results
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Specimen
2J:
embryonic day 10.5
(more  )
Note: This is a horizontal section.
Specimen
2J:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a horizontal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
2J
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Expression is present in motoneurons.
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| TS17: rhombomere 1 |
Present |
Regionally restricted |
2J
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Expression is present in motoneurons.
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Specimen
2L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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Smotm2Amc/Smotm2Amc
En1tm2(cre)Wrst/En1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant. This is a horizontal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
2L
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The dorsal population of motoneurons was present in the mutants but not the ventral one.
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| TS17: rhombomere 1 |
Present |
Regionally restricted |
2L
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The dorsal population of motoneurons was present in the mutants but not the ventral one.
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Specimen
2N:
embryonic day 12.5
(more )
Note: This is a horizontal section.
Specimen
2N:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a horizontal section.
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Specimen
2P:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL * Swiss Webster
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Mutant Allele(s):
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Smotm1Amc/Smotm2Amc
Tg(Nes-cre)1Kln/0
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant. This is a horizontal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cerebellum primordium |
Present |
Regionally restricted |
2P
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Expression is present in ventral neurons, although they were slightly reduced in number compared with wild type.
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| TS20: midbrain |
Present |
Regionally restricted |
2P
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Expression is present in ventral neurons, although they were slightly reduced in number compared with wild type.
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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Smotm2Amc/Smotm2Amc
En1tm2(cre)Wrst/En1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
Present |
Regionally restricted |
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The dorsal population of motoneurons was present in the mutants but not the ventral one.
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| TS20: cerebellum primordium |
Present |
Regionally restricted |
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The dorsal population of motoneurons was present in the mutants but not the ventral one.
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
Absent |
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There is no expression in the ventral mesencephalon.
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| TS26: cerebellum |
Absent |
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There is no expression in rhombomere 1.
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Analysis Tools