Gene Expression Data
Assay Details
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Assay
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Reference:
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J:11893
Chapman DL, et al., Isolation of the murine cyclin B2 cDNA and characterization of the lineage and temporal specificity of expression of the B1 and B2 cyclins during oogenesis, spermatogenesis and early embryogenesis. Development. 1993 May;118(1):229-40
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3809777
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Gene symbol:
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Ccnb2
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Gene name:
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cyclin B2
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Probe:
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p3.3
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Probes were hydrolyzed to 0.1 kb.
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Results
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Specimen
3A,B:
postnatal adult; at/ at
(more  )
Note: Bright-field and epiluminescence.
Specimen
3A,B:
(close  )
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Genetic Background:
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involves: DBA/1oHu
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Mutant Allele(s):
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at/at
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bright-field and epiluminescence.
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Specimen
3C,D:
postnatal adult; at/at +
(more )
Note: Bright-field and epiluminescence.
Specimen
3C,D:
(close )
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Genetic Background:
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involves: DBA/1oHu
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Mutant Allele(s):
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at/at+
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bright-field and epiluminescence.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: seminiferous tubule |
Present |
Regionally restricted |
3C/D
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Expression is in stage I tubules, absent in somatic cells.
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| TS28: spermatid |
Present |
Regionally restricted |
3C/D
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Expression is in the early round spermatids in the middle layer of the tubule and is absent in the elongating spermatids in the adluminal region.
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| TS28: spermatocyte |
Present |
Regionally restricted |
3C/D
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Expression is absent in permatocytes that have just entered the pachytene stage of meiosis I (see Fig. 3E-H fore more expression).
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Specimen
3E,F:
postnatal adult; at/at +
(more )
Note: Bright-field and epiluminescence.
Specimen
3E,F:
(close )
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Genetic Background:
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involves: DBA/1oHu
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Mutant Allele(s):
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at/at+
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bright-field and epiluminescence.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: seminiferous tubule |
Present |
Regionally restricted |
3E/F
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Stage IV tubule, absent in somatic cells.
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| TS28: spermatocyte |
Weak |
Regionally restricted |
3E/F
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Expression is in spermatocytes that had reached the mid-pachytene stage, absent in the preleptotene spermatocytes.
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| TS28: spermatid |
Present |
Regionally restricted |
3E/F
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Expression is absent in the elongating spermatids in the adluminal region.
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Specimen
3G,H:
postnatal adult; at/at +
(more )
Note: Bright-field and epiluminescence.
Specimen
3G,H:
(close )
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Genetic Background:
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involves: DBA/1oHu
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Mutant Allele(s):
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at/at+
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bright-field and epiluminescence.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: seminiferous tubule |
Present |
Regionally restricted |
3G/H
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Stage X-XI tubules, absent in somatic cells.
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| TS28: spermatocyte |
Present |
Regionally restricted |
3G/H
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Expression is in pachytene and diplotene spermatocytes, absent in leptotene and zygotene of meiosis I.
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| TS28: spermatid |
Present |
Regionally restricted |
3G/H
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Expression is absent in the elongating spermatids in the adluminal region.
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Specimen
Not shown:
postnatal day 7; at/at +
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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involves: DBA/1oHu
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Mutant Allele(s):
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at/at+
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Age:
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postnatal day 7
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5A,B:
postnatal day 22
(more )
Note: Bright-field and epiluminescence.
Specimen
5A,B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 22
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bright-field and epiluminescence.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: primary oocyte |
Weak |
Regionally restricted |
5A/B
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Expression was first detected at low levels in growing oocytes of follicles staged 3b and was absent in the resting oocytes.
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| TS28: ovary stratum granulosum |
Present |
Regionally restricted |
5A/B
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Expression was most abundant over those granulosa cells closest to the oocyte in particular of stage 5 follicles and larger.
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Specimen
5C,D:
postnatal adult
(more )
Note: Bright-field and epiluminescence.
Specimen
5C,D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Age Note:
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Older than 35 days.
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bright-field and epiluminescence.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: primary oocyte |
Present |
Regionally restricted |
5C/D
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Expression is in oocytes as they continued to grow through to the antral stages.
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| TS28: ovary stratum granulosum |
Present |
Regionally restricted |
5C/D
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Expression was most abundant over those granulosa cells closest to the oocyte in particular of stage 5 follicles and larger.
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Specimen
6D:
postnatal adult
(more )
Note: Epiluminescence optics.
Specimen
6D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Epiluminescence optics.
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Specimen
6E:
postnatal adult
(more )
Note: Epiluminescence optics.
Specimen
6E:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Epiluminescence optics.
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Specimen
6F:
embryonic day 0.5
(more )
Note: Epiluminescence optics.
Specimen
6F:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 0.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Epiluminescence optics.
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Specimen
7C,D:
embryonic day 3.5
(more )
Note: Bright-field and epiluminescence.
Specimen
7C,D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Bright-field and epiluminescence.
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Analysis Tools