Probe:	probe mix
Probe preparation:	Antisense labelled with P33
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: liver parenchyma	Strong		1A	Expression is strong throughout.
TS28: liver lobule	Present	Non-Uniform	1A	Outer parts of the hepatic lobules showed strong expression, while the signal decreased towards the central lobular area.

Structure	Level	Pattern	Image	Note
TS28: liver	Present		1A inset	Expression is present in hepatocytes.

Structure	Level	Pattern	Image	Note
TS28: renal cortex	Moderate	Non-Uniform	1B	Expression is concentrated in distinct focal areas in the juxtamedullary cortex.

Structure	Level	Pattern	Image	Note
TS28: cortical collecting duct	Present		1B inset	Expression is present in the epithelial cells of collecting ducts.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: seminiferous tubule		Present	Regionally restricted	1C	There is expression in the seminiferous tubules, although there is no expression in the Sertoli cells.
TS28: Sertoli cell	Sertoli cell	Absent		1C	There is expression in the seminiferous tubules, although there is no expression in the Sertoli cells.
TS28: Leydig cell	Leydig cell	Absent		1C

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		1C inset
TS28: spermatogonium	Absent		1C inset

Structure	Level	Pattern	Image	Note
TS28: tongue epithelium	Present		1D	Expression is concentrated in the stratified squamous epithelium.

Structure	Level	Pattern	Image	Note
TS28: tongue	Present	Regionally restricted	1D inset	Expression is present in cells of the stratum spinosum, whereas there appears to be no expression in cells of the stratum basale.

Structure	Level	Pattern	Image	Note
TS28: trigeminal ganglion	Present	Regionally restricted	1E	Expression is present in the neurons but not the glia.

Structure	Level	Pattern	Image	Note
TS28: trigeminal ganglion	Present		1E inset

Structure	Level	Pattern	Image	Note
TS28: retina	Very strong		1F
TS28: cornea	Moderate		1F	Expression levels were weak to moderate.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		1F inset
TS28: retina inner nuclear layer	Present		1F inset	Expression is present in bipolar cells.
TS28: photoreceptor layer	Absent		1F inset
TS28: pigmented retinal epithelium	Absent		1F inset

Structure	Level	Pattern	Image	Note
TS28: metanephros	Weak			Expression levels vary from low to very low.
TS28: adrenal gland	Absent
TS28: gut	Absent
TS28: heart	Absent
TS28: lymph node	Absent
TS28: skeletal muscle	Absent
TS28: smooth muscle tissue	Absent
TS28: spleen	Absent
TS28: thymus	Absent
TS28: dorsal root ganglion	Present	Regionally restricted		Expression is present in the neurons, not the glia.
TS28: superior cervical ganglion	Present
TS28: esophagus	Moderate
TS28: skin	Moderate
TS28: salivary gland	Present	Single cells		Expression is seen in a few cells between the secretory acini.

Structure	Level	Pattern	Image	Note
TS28: mammary gland	Absent
TS28: ovary	Absent

Structure	Level	Pattern	Image	Note
TS28: seminal vesicle	Absent

Structure	Level	Pattern	Image	Note
TS28: frontal cortex	Present		2A
TS28: insular cortex	Present	Regionally restricted	2A	Expression is present in the agranular insular cortex.
TS28: anterior olfactory nucleus	Very strong		2A
TS28: taenia tecta	Present		2A

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Strong	Non-Uniform	2B	Expression is high in the telenephalon, with the exception of the striatum. This includes the different fields of the neocortex, archicortex, and paleocortex.
TS28: indusium griseum	Present		2B
TS28: lateral septal nucleus	Present		2B
TS28: accumbens nucleus	Absent		2B
TS28: caudate-putamen	Absent		2B
TS28: corpus striatum	Absent		2B
TS28: globus pallidus	Absent		2B
TS28: nucleus of diagonal band	Present		2B	Expression is present in the horizontal and ventral limb of the diagonal band.
TS28: piriform cortex	Present		2B

Structure	Level	Pattern	Image	Note
TS28: lateral septal nucleus	Present		2C	Expression is present in the laterodorsal septal nucleus.
TS28: septohippocampal nucleus	Present		2C
TS28: bed nucleus of stria terminalis	Present		2C
TS28: nucleus of diagonal band	Present		2C	Expression is present in the horizontal limb of the diagonal band.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Present		2D
TS28: anterior dorsal thalamic nucleus	Present		2D
TS28: reticular thalamic nucleus	Present		2D
TS28: anterior ventral thalamic nucleus	Present		2D

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present		2E
TS28: hippocampus CA2	Present		2E
TS28: hippocampus CA3	Present		2E
TS28: medial habenular nucleus	Very strong		2E
TS28: ventral posterior lateral thalamic nucleus	Present		2E
TS28: zona incerta	Present		2E
TS28: dorsomedial hypothalamic nucleus	Present		2E
TS28: ventromedial hypothalamic nucleus	Strong		2E
TS28: arcuate nucleus	Present		2E
TS28: basomedial amygdaloid nucleus	Present		2E
TS28: basolateral amygdaloid nucleus	Present		2E
TS28: dentate gyrus	Present		2E
TS28: amygdala	Strong	Non-Uniform	2E	Expression strong in the amygdalar complex, except in the central amygdala, where it is weak.
TS28: mammillary body	Present		2E
TS28: midbrain	Present		2E

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Present	Regionally restricted	2F	Expression is present in the retrosplenial cortex.
TS28: occipital cortex	Present		2F
TS28: temporal lobe	Present		2F
TS28: superior colliculus	Present		2F
TS28: periaqueductal grey	Present		2F	Expression is present in the central gray.
TS28: medial geniculate nucleus	Present		2F
TS28: nucleus of darkschewitsch	Present		2F
TS28: red nucleus	Present		2F
TS28: substantia nigra pars reticulata	Present		2F
TS28: posteromedial cortical amygdaloid nucleus	Present		2F
TS28: amygdala	Strong	Non-Uniform	2F	Expression strong in the amygdalar complex, except in the central amygdala, where it is weak.
TS28: midbrain	Present		2F

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Present		2G
TS28: main olfactory bulb glomerular layer	Present		2G
TS28: main olfactory bulb granule cell layer	Absent		2G
TS28: lateral septal nucleus	Present		2G
TS28: anterior dorsal thalamic nucleus	Present	Regionally restricted	2G
TS28: bed nucleus of stria terminalis	Present		2G
TS28: zona incerta	Present		2G
TS28: lateral hypothalamic area	Present		2G
TS28: superior colliculus	Present		2G
TS28: inferior colliculus	Present		2G
TS28: red nucleus	Present		2G
TS28: periaqueductal grey	Present		2G	Expression is present in the central gray.
TS28: dorsal tegmental nucleus	Present		2G
TS28: reticular tegmental nucleus	Present		2G
TS28: cochlear nucleus	Present		2G	Expression is present in the vestibular nuclei.
TS28: inferior olivary nucleus	Present		2G
TS28: pontine nucleus	Present		2G
TS28: olfactory cortex	Present		2G
TS28: cerebellar cortex	Very strong		2G	This is where the highest labeling intensity in the adult brain was seen.
TS28: cerebellum granule cell layer	Very strong		2G

Structure	Level	Pattern	Image	Note
TS28: diencephalon	Present	Non-Uniform		Expression in the adult diencephalic nuclei varied from weak to very strong.
TS28: brainstem nucleus	Present	Non-Uniform		Expression in the adult brainstem nuclei varied from weak to very strong.
TS28: telencephalon	Present	Non-Uniform		Several telencephalic nuclei were moderately to strongly labeled.
TS28: neocortex layer	Present	Uniform		Expression was evenly distributed through the cortical layers.
TS28: brain nucleus	Present	Non-Uniform		In the cerebellar deep nuclei, expression was detected in a subset of large neurons.
TS28: spinal cord gray matter	Strong			Expression in the spinal cord is restricted to the gray matter.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Very strong
TS28: main olfactory bulb glomerular layer	Strong
TS28: olfactory tubercle	Weak
TS28: anterior olfactory nucleus	Very strong
TS28: nucleus of lateral olfactory tract	Weak
TS28: telencephalon	Strong	Regionally restricted		Expression is present in the subplate.
TS28: claustrum	Strong
TS28: piriform cortex	Very strong
TS28: frontal cortex	Strong
TS28: cingulate cortex	Strong			This includes expression in the retrosplenial cortex.
TS28: neocortex region	Strong	Regionally restricted		Expression is present in the orbital cortex.
TS28: insular cortex	Strong
TS28: parietal cortex	Strong
TS28: temporal lobe	Strong
TS28: perirhinal cortex	Present
TS28: entorhinal cortex	Strong
TS28: occipital cortex	Strong
TS28: endopiriform nucleus	Strong
TS28: lateral septal nucleus	Present
TS28: septohippocampal nucleus	Present
TS28: nucleus of diagonal band	Strong			This includes the ventral and horizontal limbs.
TS28: bed nucleus of stria terminalis	Present
TS28: basolateral amygdaloid nucleus	Strong
TS28: basomedial amygdaloid nucleus	Strong
TS28: amygdala	Weak	Regionally restricted		Weak expression is present in the central nucleus.
TS28: medial amygdaloid nucleus	Strong
TS28: amygdalohippocampal area	Strong
TS28: posteromedial cortical amygdaloid nucleus	Strong
TS28: hippocampus CA1	Very strong
TS28: hippocampus CA2	Very strong
TS28: hippocampus CA3	Very strong
TS28: dentate gyrus	Very strong
TS28: taenia tecta	Strong
TS28: indusium griseum	Present
TS28: subiculum	Strong
TS28: medial habenular nucleus	Very strong
TS28: anterior dorsal thalamic nucleus	Strong
TS28: anterior ventral thalamic nucleus	Present
TS28: medial dorsal thalamic nucleus	Present
TS28: reticular thalamic nucleus	Present
TS28: ventral posterior medial thalamic nucleus	Very strong
TS28: ventral posterior lateral thalamic nucleus	Strong
TS28: paracentral nucleus	Present
TS28: central medial thalamic nucleus	Present
TS28: central lateral thalamic nucleus	Present
TS28: parafascicular nucleus	Present
TS28: medial geniculate nucleus	Strong
TS28: lateral geniculate nucleus	Strong
TS28: medial preoptic nucleus	Weak
TS28: ventromedial hypothalamic nucleus	Strong
TS28: dorsomedial hypothalamic nucleus	Weak
TS28: arcuate nucleus	Present
TS28: mammillary body	Strong
TS28: subthalamic nucleus	Strong
TS28: zona incerta	Present
TS28: substantia nigra pars compacta	Weak
TS28: nucleus of darkschewitsch	Present
TS28: edinger-westphal nucleus	Present
TS28: red nucleus	Present
TS28: inferior colliculus	Present
TS28: superior colliculus	Present
TS28: periaqueductal grey	Present			Expression is present in the central gray.
TS28: pontine nucleus	Very strong
TS28: reticular tegmental nucleus	Very strong
TS28: dorsal tegmental nucleus	Very strong
TS28: dorsal raphe nucleus	Weak
TS28: median raphe nucleus	Weak
TS28: inferior olivary nucleus	Very strong
TS28: medial vestibular nucleus	Present			Expression is present in the vestibular nucleus.
TS28: cochlear nucleus	Strong
TS28: nucleus ambiguus	Weak
TS28: lateral medullary reticular formation	Very strong
TS28: cerebellar cortex	Very strong
TS28: spinal cord	Very strong

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Present		3A	Expression is present in pyramidal neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		3C	Expression is present in neurons.
TS28: cerebellum molecular layer	Strong		3C inset , 3C	Expression is present in interneurons, small neurons in the vicinity of Purkinje cells.
TS28: cerebellum Purkinje cell layer	Absent		3C inset , 3C

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Strong		3E	Expression is present in neurons.

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Strong		3F	Expression is present in neurons in the V layer of frontoparietal cortex.

Structure	Level	Pattern	Image	Note
TS28: red nucleus	Strong		3G	Expression is present in the neurons.

Structure	Level	Pattern	Image	Note
TS28: lateral medullary reticular formation	Strong		3H	Expression is present in the neurons of the lateral reticular formation.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Regionally restricted	4A	There is abundant expression in neural-crest derived structures.
TS15: spinal ganglion	Present		4A	There is expression in sensory and autonomic ganglia.
TS15: neural tube	Weak		4A

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Non-Uniform		Expression levels and distribution are similar to what was seen earlier.
TS17: neural tube	Weak

Structure	Level	Pattern	Image	Note
TS20: brainstem	Strong		4B
TS20: diencephalon	Strong		4B
TS20: spinal cord	Strong		4B
TS20: corpus striatum	Weak		4B
TS20: neocortex	Weak		4B
TS20: heart	Present		4B	Expression levels vary from weak to moderate.
TS20: metanephros	Weak		4B	Expression levels vary from low to very low.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Present		4C

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Present		4D
TS27: telencephalon	Present		4D
TS27: brown fat	Weak		4D

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Strong		4F

Structure	Level	Pattern	Image	Note
TS24: brown fat	Strong		4G

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Strong		4H

Structure	Level	Pattern	Image	Note
TS22: retina	Strong