Cyp24a1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:111272 Chau H, et al., Renal calcification in mice homozygous for the disrupted type IIa Na/Pi cotransporter gene Npt2. J Bone Miner Res. 2003 Apr;18(4):644-57
Assay type:	RT-PCR
MGI Accession ID:	MGI:3805310
Gene symbol:	Cyp24a1
Gene name:	cytochrome P450, family 24, subfamily a, polypeptide 1

Probe:	24-hydroxylase-pA, 24-hydroxylase-pB
Visualized with:	Ethidium bromide

Results

Image: 8B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	135 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
E14.5	E14.5 (a)	TS22: metanephros	Absent	2 µg; total RNA	involves: 129S2/SvPas * C57BL/6J	Slc34a1^tm1Hten/Slc34a1^tm1Hten	Not Specified
E16.5	E16.5 (a)	TS24: metanephros	Present	2 µg; total RNA	involves: 129S2/SvPas * C57BL/6J	Slc34a1^tm1Hten/Slc34a1^tm1Hten	Not Specified
E18.5	E18.5 (a)	TS26: metanephros	Present (c)	2 µg; total RNA	involves: 129S2/SvPas * C57BL/6J	Slc34a1^tm1Hten/Slc34a1^tm1Hten	Not Specified
NEWBORN	P1-5	TS27: metanephros	Present (c)	2 µg; total RNA	involves: 129S2/SvPas * C57BL/6J	Slc34a1^tm1Hten/Slc34a1^tm1Hten	Not Specified
WEANLING	P21-25	TS28: metanephros	Present (c)	2 µg; total RNA	involves: 129S2/SvPas * C57BL/6J	Slc34a1^tm1Hten/Slc34a1^tm1Hten	Not Specified
ADULT	P adult	TS28: metanephros	Present (c)	2 µg; total RNA	involves: 129S2/SvPas * C57BL/6J	Slc34a1^tm1Hten/Slc34a1^tm1Hten	Not Specified
LIVER	P adult	TS28: liver	Absent	2 µg; total RNA	involves: 129S2/SvPas * C57BL/6J	Slc34a1^tm1Hten/Slc34a1^tm1Hten	Not Specified
-RT	Control

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) The developmental expression pattern was similar between wild type and mutant.
(c) Expression was highest at E18.5 and in the postnatal period.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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