Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:111272
Chau H, et al., Renal calcification in mice homozygous for the disrupted type IIa Na/Pi cotransporter gene Npt2. J Bone Miner Res. 2003 Apr;18(4):644-57
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:3805218
|
|
Gene symbol:
|
Spp1
|
|
Gene name:
|
secreted phosphoprotein 1
|
|
Antibody:
|
anti-OPN LF-123
|
|
Detection system:
|
Secondary antibody coupled to colloidal gold
|
|
Assay notes:
|
The fixative was 4% glutaraldehyde/1% paraformaldehyde and the specimen was embedded in LR white resin.
|
|
|
Results
|
Specimen
5J:
(close  )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
|
Mutant Allele(s):
|
Slc34a1tm1Hten/Slc34a1tm1Hten
|
|
Age:
|
postnatal day 21-25
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Resin/plastic
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: metanephros |
Present |
Regionally restricted |
5J
|
Expression was detected in the calcified deposit. It was most intense at the periphery of the deposit.
|
|
Analysis Tools
)
