Gene Expression Data
Assay Details
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Assay
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Reference:
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J:111272
Chau H, et al., Renal calcification in mice homozygous for the disrupted type IIa Na/Pi cotransporter gene Npt2. J Bone Miner Res. 2003 Apr;18(4):644-57
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3805213
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Gene symbol:
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Spp1
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Gene name:
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secreted phosphoprotein 1
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Antibody:
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anti-OPN LF-123
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to alkaline phosphatase
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Assay notes:
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The fixative was 4% glutaraldehyde/1% paraformaldehyde.
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Results
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Specimen
5D:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Slc34a1tm1Hten/Slc34a1tm1Hten
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Age:
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postnatal day 23
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal cortex |
Present |
Regionally restricted |
5D
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Expression was detected in mineral deposits.
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Specimen
5E:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Slc34a1tm1Hten/Slc34a1tm1Hten
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Age:
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postnatal day 23
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal medulla |
Present |
Regionally restricted |
5E
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Expression was detected in mineral deposits.
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Slc34a1tm1Hten/Slc34a1tm1Hten
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Age:
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postnatal day 23
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal papilla |
Present |
Regionally restricted |
5F
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Expression was detected in mineral deposits.
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Specimen
5G:
postnatal day 23
(more  )
Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 23
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
5H:
postnatal day 23
(more )
Specimen
5H:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 23
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
5I:
postnatal day 23
(more )
Specimen
5I:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 23
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Analysis Tools