Gene Expression Data
Assay Details
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Assay
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Reference:
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J:65599
Gaufo GO, et al., Hoxb1 controls effectors of sonic hedgehog and mash1 signaling pathways. Development. 2000 Dec;127(24):5343-54
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3800526
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Gene symbol:
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Hoxb1
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Gene name:
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homeobox B1
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Reporter:
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GFP
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Direct Detection
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Results
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Specimen
2a:
embryonic day 8.5; Hoxb1tm5Mrc/Hoxb1 +
(more  )
Note: 6 somites.
Specimen
2a:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: rhombomere 3 |
Present |
Regionally restricted |
2a
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Expression was detected in the dorsal region of rhombomere 3.
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| TS13: rhombomere 4 |
Present |
Uniform |
2a
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GFP was uniformly expressed in rhombomere 4.
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| TS13: future hindbrain |
Present |
Regionally restricted |
2a
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Expression was detected in rhombomere 4 and dorsal rhombomere 3. Below rhombomere 4, a transverse band of cells does not express GFP. Expression in the posterior hindbrain region was downregulated.
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Specimen
2b:
embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1 +
(more )
Note: 21 somites.
Specimen
2b:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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21 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: rhombomere 4 |
Present |
Regionally restricted |
2b
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Expression was detected in the ventricular neuroepithelium.
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| TS15: rhombomere 4 lateral wall |
Present |
Regionally restricted |
2b
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Expression was detected in ventral and dorsal columns corresponding to regions of the basal and alar plates.
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Specimen
2c:
embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1 +
(more )
Note: 27 somites.
Specimen
2c:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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27 somites.
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Specimen
2f:
embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1 +
(more )
Note: Double labeled: green - Hoxb1; red - Shh (assay MGI:1928091).
Specimen
2f:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1+
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Hoxb1; red - Shh (assay MGI:1928091).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: rhombomere 4 mantle layer |
Present |
Regionally restricted |
2f
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Expression was detected in the ventricular and mantle layers of the neuroepithelium, juxtaposed to the floor plate.
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| TS18: rhombomere 4 ventricular layer |
Present |
Regionally restricted |
2f
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Expression was detected in the ventricular and mantle layers of the neuroepithelium, juxtaposed to the floor plate.
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| TS18: rhombomere 4 marginal layer |
Present |
Regionally restricted |
2f
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A small population of GFP-expressing neurons was visible in the marginal layer that contained more differentiated neurons.
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Specimen
2g:
embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1 +
(more )
Note: Double labeled: green - Hoxb1; red - Shh (assay MGI:1928091).
Specimen
2g:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1+
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Hoxb1; red - Shh (assay MGI:1928091).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: rhombomere 5 mantle layer |
Present |
Regionally restricted |
2g
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Expression was detected in a distinct cluster in the mantle layer immediately lateral to the ventral progenitor domain (VPD). The VPD is a region containing progenitor cells juxtaposed to the floor plate.
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Specimen
2h:
embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1 +
(more )
Note: Double labeled: green - Hoxb1; red - Shh (assay MGI:1928091).
Specimen
2h:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1+
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Hoxb1; red - Shh (assay MGI:1928091).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: rhombomere 5 mantle layer |
Present |
Regionally restricted |
2h
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Expression was detected in a distinct cluster in the mantle layer immediately lateral to the ventral progenitor domain (VPD). The VPD is a region containing progenitor cells juxtaposed to the floor plate.
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Specimen
2i:
embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1 +
(more )
Note: Double labeled: green - Hoxb1; red - Shh (assay MGI:1928091).
Specimen
2i:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1+
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Hoxb1; red - Shh (assay MGI:1928091).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: rhombomere 6 marginal layer |
Present |
Regionally restricted |
2i
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Expression was detected in a ventrolateral position in the marginal layer of the neuroepithelium.
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Specimen
2j:
embryonic day 12.5; Hoxb1tm5Mrc/Hoxb1 +
(more )
Note: Ventricular view.
Specimen
2j:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1+
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Ventricular view.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
2j
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Expression was detected in rhombomere 4-specific neuronal columns.
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| TS20: hindbrain mantle layer |
Present |
Regionally restricted |
2j
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Expression was detected in the posterior migrating neurons of the facial nucleus located in the deeper mantle layer.
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Specimen
2k:
embryonic day 12.5; Hoxb1tm5Mrc/Hoxb1 +
(more )
Note: Pial view.
Specimen
2k:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1+
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Pial view.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
2k
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Expression was detected in migrating rhombomere 4-derived neurons. The facial nucleus and the axonal projections of rhombomere 4-derived neurons are visible, as well as commissural axons traversing at the level of the floor plate.
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Specimen
not shown:
embryonic day 14.5; Hoxb1tm5Mrc/Hoxb1 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxb1tm5Mrc/Hoxb1+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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Not Specified
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