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Gene Expression Data
Assay Details
Assay
Reference: J:65599 Gaufo GO, et al., Hoxb1 controls effectors of sonic hedgehog and mash1 signaling pathways. Development. 2000 Dec;127(24):5343-54
Assay type: Immunohistochemistry
MGI Accession ID: MGI:1928091
Gene symbol: Shh
Gene name: sonic hedgehog
Antibody: 5E1
Detection system: Secondary antibody coupled to Texas Red
Results
Specimen 2f: embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1+ (more )
Note: Double labeled: red - Shh; green - Hoxb1 (assay MGI:3800526). Theiler stage 18 was chosen to describe rhombomere expression.
Structure Level Pattern Image Note
TS18: rhombomere 4 floor plate Present 2f
Specimen 2g: embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1+ (more )
Note: Double labeled: red - Shh; green - Hoxb1 (assay MGI:3800526). Theiler stage 18 was chosen to describe rhombomere expression.
Structure Level Pattern Image Note
TS18: rhombomere 5 floor plate Present 2g
Specimen 2h: embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1+ (more )
Note: Double labeled: red - Shh; green - Hoxb1 (assay MGI:3800526). Theiler stage 18 was chosen to describe rhombomere expression.
Structure Level Pattern Image Note
TS18: rhombomere 5 floor plate Present 2h
Specimen 2i: embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1+ (more )
Note: Double labeled: red - Shh; green - Hoxb1 (assay MGI:3800526). Theiler stage 18 was chosen to describe rhombomere expression.
Structure Level Pattern Image Note
TS18: rhombomere 6 floor plate Present 2i
Specimen 3a: embryonic day 11.5 (more )
Note: Double labeled: red - Shh; blue - PHH3. Theiler stage 18 was chosen to describe rhombomere expression.
Structure Level Pattern Image Note
TS18: rhombomere 4 floor plate Present 3a
Specimen 3b: embryonic day 11.5; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Note: Double labeled: red - Shh; blue - PHH3. Theiler stage 18 was chosen to describe rhombomere expression.
Structure Level Pattern Image Note
TS18: rhombomere 4 floor plate Present 3b At this level of analysis, it is difficult to detect appreciable changes in exprtession between wild-type and mutant mice.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory