Gene Expression Data
Assay Details
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Assay
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Reference:
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J:130238
Tsutsumi T, et al., Disruption of the Fbxw8 gene results in pre- and postnatal growth retardation in mice. Mol Cell Biol. 2008 Jan;28(2):743-51
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3776753
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Gene symbol:
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Fbxw8
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Gene name:
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F-box and WD-40 domain protein 8
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
3C:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Fbxw8Gt(RRT057)Byg/Fbxw8Gt(RRT057)Byg
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: skeletal muscle |
Strong |
Non-Uniform |
3C
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There is high expression in the skeletal muscle, with intense staining observed in the intercostals and abdominal walls. Muscles in the extremities are also stained.
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| TS26: diaphragm |
Very strong |
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3C
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| TS26: smooth muscle tissue |
Absent |
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3C
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There is slight, if any, expression in the smooth muscle.
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Fbxw8Gt(RRT057)Byg/Fbxw8Gt(RRT057)Byg
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: skeleton |
Present |
Regionally restricted |
3E
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Expression is present in bone, especially in the epiphysis of tubular bone corresponding to cartilage. It is not present in the diaphysis, where the formation of bone has occured.
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Fbxw8Gt(RRT057)Byg/Fbxw8Gt(RRT057)Byg
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: skeleton |
Present |
Regionally restricted |
3F
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Expression is present in bone, especially in the epiphysis of tubular bone corresponding to cartilage. It is not present in the diaphysis, where the formation of bone has occured.
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Specimen
3G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Fbxw8Gt(RRT057)Byg/Fbxw8Gt(RRT057)Byg
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Fbxw8Gt(RRT057)Byg/Fbxw8Gt(RRT057)Byg
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: placenta |
Present |
Regionally restricted |
3H
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Expression is intense in the labyrinthine layer and moderate in the spongiotrophoblast layer.
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| TS28: decidua |
Absent |
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3H
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Specimen
3I:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Fbxw8Gt(RRT057)Byg/Fbxw8Gt(RRT057)Byg
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: placenta |
Present |
Regionally restricted |
3I
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Expression is intense in the labyrinthine layer and moderate in the spongiotrophoblast layer.
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| TS28: decidua |
Absent |
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3I
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Fbxw8Gt(RRT057)Byg/Fbxw8Gt(RRT057)Byg
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Fbxw8Gt(RRT057)Byg/Fbxw8+
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: embryo |
Present |
Regionally restricted |
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The expression patterns are similar to those seen in -/- animals, but the levels of expression are reduced.
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Fbxw8Gt(RRT057)Byg/Fbxw8Gt(RRT057)Byg
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: embryo |
Present |
Regionally restricted |
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The expression patterns are similar to those seen at E18.5.
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Analysis Tools
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