Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Shhtm6Amc
Name:	sonic hedgehog; targeted mutation 6, Andrew P McMahon
MGI ID:	MGI:3775846
Synonyms:	Shh::GFP
Gene:	Shh  Location: Chr5:28661838-28672099 bp, - strand  Genetic Position: Chr5, 14.39 cM
Alliance:	Shhtm6Amc page

Germline Transmission:	Earliest citation of germline transmission: J:132152
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129X1/SvJ

Allele Type:    Targeted (Null/knockout, Reporter) Mutation:    Insertion   Mutation details: A GFP cassette was inserted into exon 3 and an FRT site flanked neomycin resistance gene cassette was inserted between exon 2 and 3. The presence of the fusion protein and the absence of the endogenous product was confirmed by western blot analysis on E9.5 embryo extracts. This allele produces functional albeit inefficient protein product. (J:132152)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

5 assay results

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Shh Mutation:	45 strains or lines available

Original:	J:132152 Chamberlain CE, et al., Notochord-derived Shh concentrates in close association with the apically positioned basal body in neural target cells and forms a dynamic gradient during neural patterning. Development. 2008 Mar;135(6):1097-106
All:	6 reference(s)