Summary | Transgene origin | Transgene description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Tg(CAG-SAC/EGFP)35Rang
Name:	transgene insertion 35, Vivek Rangnekar
MGI ID:	MGI:3772442
Synonyms:	SAC/35, SAC-GFP line 35, Tg(ACTB-SAC/EGFP)35Rang
Transgene:	Tg(CAG-SAC/EGFP)35Rang  Location: unknown
Alliance:	Tg(CAG-SAC/EGFP)35Rang page

Strain of Origin:

(C57BL/6 x C3H)F1

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Mutation details: The pCA/SAC-GFP transgene was designed with a chicken beta-actin promoter (and cytomegalovirus (CMV) enhancer) upstream of a fusion gene containing a Par-4 SAC domain (Pawr gene, amino acids 137-195) cDNA fused to an enhanced green fluorescent protein (EGFP), all followed by a polyA sequence. The resulting transgenic offspring were bred to (C57BL/6 x C3H)F1 mice. Founder line 35 mice (SAC/35; found to have 6-8 copies of the transgene). Widespread expression of the transgene is observed in all tested tissues (with some differential tissue-specific regulation of transgene expression or protein stability reported). EGFP expression level is appropriate for immunoblots, but not sufficient enough for fluorescence of flow cytometry applications. (J:125633)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:125633 Zhao Y, et al., Cancer resistance in transgenic mice expressing the SAC module of Par-4. Cancer Res. 2007 Oct 1;67(19):9276-85
All:	1 reference(s)