Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:125766
Biris KK, et al., Mouse Ripply2 is downstream of Wnt3a and is dynamically expressed during somitogenesis. Dev Dyn. 2007 Nov;236(11):3167-72
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:3772238
|
Gene symbol:
|
Ripply2
|
Gene name:
|
ripply transcriptional repressor 2
|
Probe:
|
Ripply2 cDNA1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
1A:
embryonic day 7.75
(more )
Note: Headfold stage.
Specimen
1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.75
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Headfold stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: trunk paraxial mesenchyme |
Present |
Regionally restricted |
1A
|
Expression is as a single bilateral stripe located anterior and perpendicular to the node.
|
Specimen
1B:
embryonic day 8.5
(more )
Specimen
1B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1C:
embryonic day 9.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1D:
embryonic day 9.5
(more )
Note: High-magnification image of the dorsal/posterior region of the E9.5 embryo shown in C.
Specimen
1D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
High-magnification image of the dorsal/posterior region of the E9.5 embryo shown in C.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Very strong |
Regionally restricted |
1D
|
Expression is restricted to the anterior presomitic mesoderm as an intense prospective somite-wide stripe of expression in S-I.
|
Specimen
1E:
embryonic day 9.5
(more )
Specimen
1E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
1E
|
Expression is restricted to the anterior presomitic mesoderm and is as two stripes in prospective somites: a stripe in S-I and a weaker stripe in the rostral half of S0.
|
Specimen
1F:
embryonic day 10.5
(more )
Specimen
1F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1G:
embryonic day 10.5
(more )
Specimen
1G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: tail unsegmented mesenchyme |
Very strong |
Regionally restricted |
1G
|
Expression is restricted to the anterior presomitic mesoderm as an intense prospective somite-wide stripe of expression in S-I.
|
Specimen
1H:
embryonic day 12.5
(more )
Specimen
1H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: tail unsegmented mesenchyme |
Very strong |
Regionally restricted |
1H
|
Expression is restricted to the anterior presomitic mesoderm as an intense prospective somite-wide stripe of expression in S-I.
|
Specimen
1I:
embryonic day 8.5
(more )
Note: 5-6 somites.
Specimen
1I:
(close )
Genetic Background:
|
involves: 129S1/Sv
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
5-6 somites.
|
Specimen
1J:
(close )
Genetic Background:
|
involves: 129S1/Sv
|
Mutant Allele(s):
|
Wnt3atm1Amc/Wnt3atm1Amc
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
5-6 somites.
|
Specimen
2A:
embryonic day 9.5
(more )
Note: Double labeled: purple - Ripply2; orange - Uncx (assay MGI:3772243).
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Ripply2; orange - Uncx (assay MGI:3772243).
|
Specimen
2B:
embryonic day 9.5
(more )
Note: Double labeled: purple - Ripply2; orange - Tcf15 (assay MGI:3772241).
Specimen
2B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Ripply2; orange - Tcf15 (assay MGI:3772241).
|
Specimen
2C:
embryonic day 9.5
(more )
Note: Double labeled: purple - Ripply2; orange - T (assay MGI:3772240).
Specimen
2C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Ripply2; orange - T (assay MGI:3772240).
|
Specimen
2D:
embryonic day 9.5
(more )
Note: Double labeled: purple - Ripply2; orange - Dll1 (assay MGI:3772242).
Specimen
2D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Ripply2; orange - Dll1 (assay MGI:3772242).
|
Specimen
2E:
embryonic day 9.5
(more )
Note: Double labeled: purple - Ripply2; orange - Lfng (assay MGI:3772245).
Specimen
2E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Ripply2; orange - Lfng (assay MGI:3772245).
|
Specimen
2F:
embryonic day 9.5
(more )
Note: Double labeled: purple - Ripply2; orange - Mesp2 (assay MGI:3772244).
Specimen
2F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Ripply2; orange - Mesp2 (assay MGI:3772244).
|
Specimen
2G:
embryonic day 9.5
(more )
Note: Double labeled: purple - Ripply2; orange - Hes7 (assay MGI:3772246).
Specimen
2G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Ripply2; orange - Hes7 (assay MGI:3772246).
|
Specimen
2H:
embryonic day 9.5
(more )
Note: Double labeled: purple - Ripply2; orange - Uncx (assay MGI:3772243).
Specimen
2H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Ripply2; orange - Uncx (assay MGI:3772243).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
2H
|
Expression is restricted to the anterior presomitic mesoderm as a somite-wide stripe in S-I.
|
Specimen
2I:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Hes7tm1Kag/Hes7tm1Kag
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: purple - Ripply2; orange - Uncx (assay MGI:3772243).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Weak |
Regionally restricted |
2I
|
Expression is restricted to the anterior presomitic mesoderm with poorly defined boundaries compared with wild-type embryos.
|
|