Gene Expression Data
Assay Details
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Assay
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Reference:
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J:110717
Hirano M, et al., A new serine/threonine protein kinase, Omphk1, essential to ventral body wall formation. Dev Dyn. 2006 Aug;235(8):2229-37
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3771064
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Gene symbol:
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Nuak1
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Gene name:
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NUAK family, SNF1-like kinase, 1
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fixative was 0.2% formalin, 0.2% glutaraldehyde.
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Results
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Specimen
3a:
embryonic day 7.5; Nuak1tm1Sia/Nuak1 +
(more  )
Specimen
3a:
(close  )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3b:
embryonic day 7.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
3b:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3c:
embryonic day 7.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
3c:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
3e:
embryonic day 8.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
3e:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3g:
embryonic day 9.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
3g:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3h:
embryonic day 9.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
3h:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
3i:
embryonic day 9.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
3i:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
3j:
embryonic day 9.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
3j:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
3k:
embryonic day 12.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
3k:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
3l:
embryonic day 14.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
3l:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: embryo |
Present |
Regionally restricted |
3l
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Expression is in a variety of connective tissues.
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| TS22: epidermis |
Present |
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3l
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| TS22: cerebral cortex |
Present |
Regionally restricted |
3l
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Expression is in differentiated fields of the cortex.
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| TS22: diencephalon |
Absent |
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3l
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No significant expression was found in other parts of brain or in the spinal cord.
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| TS22: hindbrain |
Absent |
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3l
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No significant expression was found in other parts of brain or in the spinal cord.
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| TS22: midbrain |
Absent |
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3l
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No significant expression was found in other parts of brain or in the spinal cord.
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| TS22: spinal cord |
Absent |
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3l
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No significant expression was found in other parts of brain or in the spinal cord.
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Specimen
3m:
embryonic day 18.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
3m:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: embryo |
Present |
Regionally restricted |
3m
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Expression is in a variety of connective tissues.
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| TS26: epidermis |
Present |
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3m
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| TS26: cerebral cortex |
Present |
Regionally restricted |
3m
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Expression is in differentiated fields of the cortex.
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| TS26: diencephalon |
Absent |
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3m
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No significant expression was found in other parts of brain or in the spinal cord.
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| TS26: hindbrain |
Absent |
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3m
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No significant expression was found in other parts of brain or in the spinal cord.
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| TS26: midbrain |
Absent |
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3m
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No significant expression was found in other parts of brain or in the spinal cord.
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| TS26: spinal cord |
Absent |
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3m
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No significant expression was found in other parts of brain or in the spinal cord.
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Specimen
4f:
embryonic day 14.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
4f:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
4g:
embryonic day 14.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
4g:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: trunk |
Present |
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4g
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Expression is in abdominal bands.
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Specimen
4h:
embryonic day 14.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
4h:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
4j:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1tm1Sia
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: epidermis |
Present |
Regionally restricted |
4j
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Expression in this mutant is the same as in wild-type embryos.
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| TS22: sternum |
Present |
Regionally restricted |
4j
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Expression in this mutant is the same as in wild-type embryos.
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| TS22: heart |
Present |
Regionally restricted |
4j
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
4k:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1tm1Sia
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: trunk |
Present |
Regionally restricted |
4k
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Expression is different in this mutant due to its phenotype.
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Specimen
4l:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1tm1Sia
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Age:
|
embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: trunk |
Present |
Regionally restricted |
4l
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Expression is different in this mutant due to its phenotype.
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Specimen
4n:
embryonic day 9.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
4n:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
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Age:
|
embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
|
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk |
Present |
Regionally restricted |
4n
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Expression is in the primary body wall at the thoracic level.
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| TS15: heart |
Present |
Regionally restricted |
4n
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Expression is at the surface epithelium of the heart.
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Specimen
4o:
embryonic day 9.5; Nuak1tm1Sia/Nuak1 +
(more )
Specimen
4o:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1+
|
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Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk |
Present |
Regionally restricted |
4o
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Expression is in the primary body wall at the abdominal level.
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Specimen
4q:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1tm1Sia
|
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Age:
|
embryonic day 9.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: heart |
Present |
Regionally restricted |
4q
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Expression in this mutant is the same as in wild-type embryos at the thoracic level.
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| TS15: trunk |
Present |
Regionally restricted |
4q
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Expression in this mutant is the same as in wild-type embryos at the thoracic level.
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Specimen
4r:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Nuak1tm1Sia/Nuak1tm1Sia
|
|
Age:
|
embryonic day 9.5
|
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
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Sex:
|
Not Specified
|
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Type:
|
section
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Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk |
Present |
Regionally restricted |
4r
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Expression in this mutant is the same as in wild-type embryos at the abdominal level.
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Analysis Tools