Probe:	MH956 probe 290
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Absent		MH1080
TS28: main olfactory bulb external plexiform layer	Absent		MH1080
TS28: main olfactory bulb mitral cell layer	Absent		MH1080
TS28: main olfactory bulb internal plexiform layer	Absent		MH1080
TS28: main olfactory bulb granule cell layer	Absent		MH1080
TS28: cerebral cortex	Strong		MH1080
TS28: hippocampus CA1	Weak		MH1080
TS28: hippocampus CA2	Strong		MH1080
TS28: hippocampus CA3	Absent		MH1080
TS28: dentate gyrus	Strong		MH1080
TS28: corpus striatum	Strong		MH1080
TS28: telencephalon septum	Weak		MH1080
TS28: amygdala	Moderate		MH1080
TS28: thalamus	Strong		MH1080
TS28: hypothalamus	Weak		MH1080
TS28: pretectum	Weak		MH1080
TS28: superior colliculus	Weak		MH1080
TS28: inferior colliculus	Moderate		MH1080
TS28: midbrain tegmentum	Weak		MH1080
TS28: cerebellum molecular layer	Absent		MH1080
TS28: cerebellum Purkinje cell layer	Weak		MH1080
TS28: cerebellum granule cell layer	Strong		MH1080
TS28: pons	Moderate		MH1080
TS28: medulla oblongata	Moderate		MH1080

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Absent		HB456 , BA116
TS28: main olfactory bulb external plexiform layer	Absent		BA116 , HB456
TS28: main olfactory bulb mitral cell layer	Absent		HB456 , BA116
TS28: main olfactory bulb internal plexiform layer	Absent		HB456 , BA116
TS28: main olfactory bulb granule cell layer	Absent		HB456 , BA116
TS28: cerebral cortex	Strong		HB456 , BA116
TS28: hippocampus CA1	Moderate		HB456 , BA116
TS28: hippocampus CA2	Strong		HB456 , BA116
TS28: hippocampus CA3	Absent		HB456 , BA116
TS28: dentate gyrus	Strong		BA116 , HB456
TS28: corpus striatum	Strong	Single cells	BA116 , HB456
TS28: telencephalon septum	Weak		BA116 , HB456
TS28: amygdala	Moderate		BA116 , HB456
TS28: thalamus	Strong		HB456 , BA116
TS28: hypothalamus	Weak		BA116 , HB456
TS28: pretectum	Absent		BA116 , HB456
TS28: superior colliculus	Absent		HB456 , BA116
TS28: inferior colliculus	Absent		HB456 , BA116
TS28: midbrain tegmentum	Absent		HB456 , BA116
TS28: cerebellum molecular layer	Absent		BA116 , HB456
TS28: cerebellum Purkinje cell layer	Weak		BA116 , HB456
TS28: cerebellum granule cell layer	Moderate		HB456 , BA116
TS28: pons	Weak		BA116 , HB456
TS28: medulla oblongata	Weak		BA116 , HB456

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Strong	Regionally restricted	1G	Expression is restricted to the mantle layer.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Weak	Single cells		Expression is restricted to a few individual cells scattered throughout the bulb.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		2A	Expression is present in the neurons of layers 2/3. This is one of layers with the highest expression levels.
TS28: cortical layer III	Present		2A	Expression is present in the neurons of layers 2/3. This is one of layers with the highest expression levels.
TS28: cortical layer V	Present		2A	Expression is present in the neurons. This is one of the layers with the highest expression levels.
TS28: cortical layer VI	Present		2A
TS28: cortical layer IV	Present	Non-Uniform	2A	Expression is present in a scattered cell population.
TS28: cortical layer I	Present		2A	Expression is present in the molecular layer.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong	Regionally restricted	2I	There is strong expression in the preplate.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		3A
TS28: hippocampus CA2	Present		3A
TS28: hippocampus CA1	Present	Single cells	3A	Expression is only present in a few scattered cells.
TS28: hippocampus CA3	Present	Single cells	3A	Expression is only present in a few scattered cells.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		3B
TS28: hippocampus CA2	Strong		3B
TS28: hippocampus CA1	Moderate		3B

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Regionally restricted	3O	Expression is present in the marginal zone.

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Strong		4A
TS28: globus pallidus	Present		4A	Expression is much weaker than in the caudate putamen.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Strong	Single cells	4D , 4G	Expression is restricted to a small subset of striatal cells.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Strong	Single cells	4J	Expression is restricted to a small subset of striatal cells.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present	Graded	5A	Expression is present in the ventrodorsal gradient.

Structure	Level	Pattern	Image	Note
TS28: reticular thalamic nucleus	Strong		5B

Structure	Level	Pattern	Image	Note
TS28: medial thalamic group	Strong		5C	There is strong expression in the mediodorsal nucleus.

Structure	Level	Pattern	Image	Note
TS28: reticular thalamic nucleus	Strong		5D

Structure	Level	Pattern	Image	Note
TS22: midbrain mantle layer	Strong	Regionally restricted	6A	Expression is present in a medial and dorsal longitudinal stripe in the mantle layer of the midbrain roof. It is also expressed more laterally in the mantle layer but fades with increasing distance from the midline.

Structure	Level	Pattern	Image	Note
TS22: retina	Strong