Probe:	probe A
Probe preparation:	Antisense labelled with S35 RNA
Assay notes:	Authors state that probe A (MGI:12544) was used in most experiments, but that probe B (MGI:12543) may also have been used. Both displayed the same expression patterns.

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		3A-4.8

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Regionally restricted	3B	Expression restricted to posterior areas of embryo.
TS15: embryo mesenchyme	Present	Regionally restricted	3B	Expression widely distributed in presomitic and lateral mesoderm, neural plate and hindgut, up to the cells contacting the allantois.
TS15: hindgut	Present		3B	Expression widely distributed in presomitic and lateral mesoderm, neural plate and hindgut, up to the cells contacting the allantois.
TS15: tail neural plate	Present		3B	Expression widely distributed in presomitic and lateral mesoderm, neural plate and hindgut, up to the cells contacting the allantois.
TS15: tail unsegmented mesenchyme	Present		3B	Expression widely distributed in presomitic and lateral mesoderm, neural plate and hindgut, up to the cells contacting the allantois.

Structure	Level	Pattern	Image	Note
TS19: tail somite	Present	Regionally restricted	4A	Boundary of expression along the very posterior tail somites, which are still epithelialized.
TS19: tail neural tube	Present		4A

Structure	Level	Pattern	Image	Note
TS20: tail	Weak	Regionally restricted	4B
TS20: tail paraxial mesenchyme	Present	Regionally restricted	4B	Authors report a boundary of expression between prevertebra 32 and 33.
TS20: genital tubercle	Strong		4B

Structure	Level	Pattern	Image	Note
TS24: rectum	Present	Regionally restricted	5A-4.8	Expression throughout rectum, with a sharp boundary at the rectum-anus boundary. Expression in both mesenchyme and epithelium.
TS24: uterine horn	Present	Regionally restricted	5A-4.8	Expression in the posterior part of the uterus uterus, with a well-defined anterior boundary in the uterus.
TS24: bladder wall	Present		5A-4.8

Structure	Level	Pattern	Image	Note
TS24: appendix epididymis	Present	Regionally restricted		Expression in a limited part of the epididymis.
TS24: bladder wall	Present

Structure	Level	Pattern	Image	Note
TS25: rectum	Present	Regionally restricted	5B	Restricted expression in the rectum wall.
TS25: rectum epithelium	Present	Regionally restricted	5B	Expression is more intense in the crypts of Lieberkuhn than in apical cells.
TS25: rectum mesenchyme	Present	Regionally restricted	5B	Expression in a concentric cell layer which may correspond to the muscularis.

Structure	Level	Pattern	Image	Note
TS24: rectum epithelium	Present		5C	Expression in glandular epithelium in the rectum.
TS24: anal canal epithelium	Absent		5C	No expression in malpighian anal epithelium.

Structure	Level	Pattern	Image	Note
TS20: urogenital ridge	Absent		6-4.8	No expression in the genital ridges.
TS20: gonad	Absent		6-4.8
TS20: metanephros	Absent		6-4.8

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Weak	Regionally restricted	7A	Weak expression in posterior cells of forelimb.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	7B (left)	Spatially restricted to more distal and posterior areas than Hox4.7 (Hoxd12).

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present	Regionally restricted	7C (left)	Spatially restricted to more distal and posterior areas than Hox4.7 (Hoxd12).

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present
TS19: hindlimb bud mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS20: foot mesenchyme	Strong	Regionally restricted	7D	Strong expression in the limbs, distally restricted to footplate mesenchyme
TS20: hand mesenchyme	Strong	Regionally restricted	7D	Strong expression in the limbs, distally restricted to footplate mesenchyme

Structure	Level	Pattern	Image	Note
TS23: limb	Present	Regionally restricted	7E	Authors report expression in mesenchymal cells surrounding the bone anlage.