Gene Expression Data
Assay Details
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Assay
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Reference:
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J:125199
Wei P, et al., Defective vascular morphogenesis and mid-gestation embryonic death in mice lacking RA-GEF-1. Biochem Biophys Res Commun. 2007 Nov 9;363(1):106-12
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3760895
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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anti-PECAM-1
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
3A left:
embryonic day 9.5
(more  )
Specimen
3A left:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: blood vessel |
Present |
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3A +/+
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In wild-type embryos the blood vessels, such as dorsal aortae, aortic arches, cranial vessels, and intersomitic vessels, are well organized.
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| TS15: branchial arch artery |
Present |
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3A +/+
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In wild-type embryos the blood vessels, such as dorsal aortae, aortic arches, cranial vessels, and intersomitic vessels, are well organized.
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| TS15: dorsal aorta |
Present |
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3A +/+
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In wild-type embryos the blood vessels, such as dorsal aortae, aortic arches, cranial vessels, and intersomitic vessels, are well organized.
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Specimen
3A middle:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Rapgef2tm1.1Tkat/Rapgef2tm1.1Tkat
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: blood vessel |
Present |
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3A -/- middle
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The vascular morphology varies in mutants compared to wild type. Most lack major blood vessels but possess delicate, plexus-like structures at the site of dorsal aortae.
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Specimen
3A right:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Rapgef2tm1.1Tkat/Rapgef2tm1.1Tkat
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: allantois |
Strong |
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3A -/- right
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In 3 of 12 embryos examined, there was an abnormal, balloon-shaped allantois.
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| TS15: blood vessel |
Present |
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3A -/- right
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The vascular morphology varies in mutants compared to wild type. In those with the bulbous allantois, the vasculature was less severely affected, and the structures like dorsal aortae and intersomitic vessels are visible, albeit hypomorphic.
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| TS15: dorsal aorta |
Present |
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3A -/- right
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The vascular morphology varies in mutants compared to wild type. In those with the bulbous allantois, the vasculature was less severely affected, and the structures like dorsal aortae and intersomitic vessels are visible, albeit hypomorphic.
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Specimen
3B +/+:
embryonic day 9.5
(more )
Specimen
3B +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: extraembryonic blood vessel |
Present |
Regionally restricted |
3B +/+
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There is are large vitelline collecting vessels and a network of smaller vessels.
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| TS15: yolk sac |
Present |
Regionally restricted |
3B +/+
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There is are large vitelline collecting vessels and a network of smaller vessels.
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Specimen
3B -/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Rapgef2tm1.1Tkat/Rapgef2tm1.1Tkat
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: extraembryonic blood vessel |
Present |
Regionally restricted |
3B -/-
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Expression is present in the vascular endothelium, but it fails to coalesce to form a honeycomb-like primitive vascular plexus.
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| TS15: yolk sac |
Present |
Regionally restricted |
3B -/-
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Expression is present in the vascular endothelium, but it fails to coalesce to form a honeycomb-like primitive vascular plexus.
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Analysis Tools
